The Effects Of Direct Current Electric Fields On Migration And CXCR4Expression Of Bmscs In Vitro

Adult animal digestive tract, such as muscle, fat, bone marrow, brainand other tissues and organs have multilineage differentiation potential andself-renewal of stem cells.These cells in vitro and in vivo in suitableconditions may be to a variety of cell differentiation.Bone marrowmesenchymal stem cells (BMSCs) is a kind of adult stem cells. Because ofits convenient, easy expansion and has been widely used in regenerativemedicine treatment.In addition, during embryonic development process andthe damage area exists in DC electric field.The presence of an electric fieldcan not only promote the organization development can chemotactic cellmigration, in vitro, current is applied to cells, can be induced by a varietyof cells to produce chemokines electric effect, both cells under the action ofcurrent to the anode and cathode movement.Although there arem cells in bone marrow, but can play the role of is very small,hard toeffectively play repair and reconstruction of functions role.Therefore, howto induce more directional BMSCs endogenous regional migration to damage increase damage area has become a stem cell population increasethe clinical curative effect of the key problem.Based on this idea the paper using physiological intensity of electricstimulation in vitro have multilineage differentiation potentialBMSCs,Observe the effects of electric fields on the migration ability andchemokine receptors on the expression of CXCR4protein,And furtherresearch on the effects of cell migration and the expression of CXCR4mechanism, so as to increase the stem cells migrate to the damaged area toincrease the damage repair capacity lays a foundation.Purpose：Through physical action (Electric field) promotes cellmigration, increased CXCR4expresstion, further study of relatedmechanism.In order to increase the damage area of stem cell numberincrease repair capacity to provide certain theory basis.Methods：1.Whole bone marrow culture acquisition in mousebone marrow by BMSCs.Flow cytometric detection of CD34, CD44,CD90, CD105expression.2.Scratch test for the detection of electric field on BMSCs migrationability of.3.Stimulation of BMSCs0min,30min,2h,6h,12h throughphysiological strength of the electric field (250mV/mm) respectively,then using Western Blot for detection of CXCR4protein expression. 4. Application of CXCR4inhibitor AMD3100, detection of BMSCsmigration.5. AKT，P-AKT, ERK1/2，P-ERK1/2were detected by Western Blot。Results:1.Through the cell culture, and for the expansion of the morestable BMSCs.Flow cytometric test showed that, BMSCs hematopoieticstem cells do not express the marker CD34, but expression of mesenchymalstem cells markers,CD44, CD90,CD105.2. Scratch test display, with electrical field stimulation time, cellmigration numbers gradually increased.3. With the stimulation of the extension of time of CXCR4proteinexpression levels,The2h,6h,12h CXCR4protein expression wassignificantly higher than that of control group (without electric fieldstimulation group), indicating that the electric field can promote theexpression of CXCR4protein.4. With CXCR4inhibitors stimulate cells found,AMD3100+6helectric group and individually compared with group AMD3100cellmigration increases apparently, AMD3100group and3h,6h without electricfield stimulation group comparison of cell migration is reduced.5.Electric field stimulation of BMSCs0,10min,20min,30min,P-AKT,P-ERK1/2expression for time dependent enhancement.Conclusion:1. Electrical field stimulation of BMSCs migration. 2.The electric field increases BMSCs expression of CXCR4protein.3. Electric field may partially reverse AMD3100on BMSCs migrationinhibition。4. Electric field to increase AKT, ERK1/2phosphorylation levels.