Expression And Significance Of TCF4in Pathologic Scar Tissue

Objective:To detect the expression of T cell factor TCF4) and Transforming growth factor β(TGF-β) in fibroblasts from hypertrophic scar and keloid and investigate the role of TCF4in the pathological mechanism of scar.Methods:The clinical specimens were divided into three groups, Group A from normal skin, Group B from hypertrophic scar, Group C from keloid. The control group is group A. The changes of TCF4and TGF-β gene were detected with RT-PCR and TCF4and TGF-β protein were detected with immunohistochemical and Western blot.Resμlts:The expression of TCF4with Immunohistochemical in group B and group C was strongly positive, weakly positive expression in group A. And the expression of TGF-β with Immunohistochemical in group B and group C was positive, and negative expression in group A. The result show statistically significant difference compared Group B and group C to group A in TCF4(P<0.01) and in TGF-β(P<0.05). The mRNA expression of TCF4in hypertrophic scar and keloid were significant higher than those in normal skins, and show statistically significant difference compared group B (0.4910±0.0472) and group C (0.7595±0.0366) to group A (0.2550±0.051)(P<0.05) and group C to group B (P<0.05). The protein expression of TCF4in hypertrophic scar and keloid were significant higher than those in normal skins, and show statistically significant difference compared group C (0.6780±0.0307) and groupB (0.3865±0.0412) to group A (0.1835±0.0192)(P<0.05) and group C to group B (P<0.05)Conclusions:The express of TCF4was abnormally higher in hypertrophic scar and keloid, by which regulate TGF-β expression. TGF-β can stimulated fibroblast proliferation, may participate the promoting of the pathological scar formation.