| Hypertrophic scars and keloids represent an aberration in the fundamental processes of wound healing. Excessive proliferating fibroblasts and overabundant wound matrix responsible for raised, red and inflexible scar tissue, which causes itching and pain, and might lead to serious functional and cosmetic problems for the patient. Over the years, we keep on searching for a reliable treatmen to hypertrophic scars and keloids, the therapeutic modalities include surgical excision, intralesional corticosteroids injections, radiationtherapy, laser surgery, cryotherapy and other medication, but unfortunately have not yet been successful in preventing and treating hypertrophic scar and keloid formation. The molecular weight of Matrine is 248.37 and the molecular formula is CisHjANjO which has a significant pharmaceutical effect on central nervous system, cardiovascular system, anti-inflamation, anti-tumor, anti-virus and anti-fibrosis etc. In recent years, Radices sophorae flavescentis alkaloid has been found lots of pharmaceutical activity and clinical function during the studying and using process in home and abroad, Radices sophorae flavescentis alkaloid is a kind of alkaloids which has a similar chemical constitution represented by Matrine that is also the main ingredient of scar soften ointment used in our department for many years. It can make it smaller and softer than the untreated but the mechanism is unclear.First, we studied the influence of Matrine on the fibroblasts derived from pathologic human scar in vitro. At different concentration gradient of Matrine,the MTT OD value of the fibroblasts was used for caculating the rate of proliferating inhinitation, the PCNA was tested for the expressions of proliferating cell nuclear antigen (PCNA) which performed by immunohistochemical method, the changes of the fibroblast's cell cycle was tested by flow cytometer , the apoptosic rate of early stage was tested by anti-annexin-V-FITC and anti- PI method , the ultrastructural influence of Matrine on the morphology and synthesis was performed by Transmission Electron Microscopy ( TEM). In contrast with the controls,the results showed Matrine at 0.10-100.00mg/ml could decrease the MTT OD value in dose and time dependent manner ,the PCNA was under-expressed, cell cycle showed that the percentage of G2-M phase and S phase decreased, the percentage of G0.Gi phase cells increasd significantly and the Rough endoplasmic reticulums were decreased ,the ribosomes were appeared degranulation and depolymerization ,and lysosomes were increased significantly, particularly the nuclear of chromatins were condensed to gather to the side of nuclear membranes which suggested the apoptosis of fibroblasts derived pathologic human scar.Second, we study the influence of Matrine by injection to the hypertrophic scarring model of rabbit ears to investigate the effect of matrine on hypertrophic scarring tissues in vivo. In contrast with the controls we exploit the scarring model to observe the morphologic change under visual appearance and microscope, calculated the AgNORs.dectected the expressions of proliferation cell nuclear antigen and the expression of apoptosic protein P53,Bcl-2,Bax of fibroblasts . Results showed that Matrine could inhibit the fibroblasts proliferative activity of scarring tissues, could decrease the amount of the fibroblasts of the numerical density on area and collage of Area density on area(AA) significantly, could reduce the amount of AgNORs.Matrine could make Bax over- expressed and Bcl-2, P53 ,PCNA down- expressed of the fibroblasts derived from hypertrophic scarring of the rabbit ears model respectively.So we concluded Matrine could inhibit the proliferation of the fibroblastsand accelerate the apoptosis of the fibroblastsr. Matrine have significant treatment effectiveness to decrease excessive collagen deposition and orient the scar tissue to the normal tissue in vitro and in vivo. .In general ,We first find that Matrine has the significant effectiveness to pathologic scar in vitro and in... |
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