Clinical Significance Of The Quantity Of B Lymphocyte In The Patients With Immunorelated Pancytopenia

Objective:1. Analyze the clinical significance of testing the total number of B lymphocytes and their subsets, CD5+B lymphocytes, in the bone marrow of patients with immune-related pancytopenia (IRP).2. Investigate the relationship between the immune-related whole pancytopenia (IRP) and the numbers of B lymphocytes and its subsets,CD5+B lymphocytes, in the bone marrow of the patients.3. Explore the relationship between the clinical effects of the disease treatment and the total number of B lymphocytes in bone marrow of the patients with the immune-related whole pancytopenia (IRP).Methods:Detect the total number of B lymphocytes and CD5+B lymphocytes in bone marrow by double labeling flow cytometry (FACS). The inspected object as follows:29patients were first diagnosed with the IRP (IRP diagnosis group, group A);19patients with IRP whose blood indicators restore to the normal level after treatment (complete remission after treatment, group B); and18normal ordinary people (normal control group, group C).Results:1. The percentage of B lymphocytes (CD19+cells) in bone marrow of group A, group B, and group C, occupying the bone marrow mononuclear cells (BMMNC) were(8.35±4.53)%,(2.86±1.42)%and (3.14±1.45)%. The percentage of CD5+B lymphocytes in bone marrow of group A, group B, and group C, occupying B lymphocytes (CD19+cells) were (21.19±11.53)%,(12.08±0.31)%and (11.87±5.83)%. The statistical analysis shows that:the proportions of the total number about B lymphocytes and CD5+B lymphocytes in the bone marrow of group A were significantly higher than those of group B and group C, and there is significant difference (P<0.05)between group A and group B or between group A and group C, but no significant difference (P>0.05)between group B and group C.2. The percentage of B lymphocytes in the bone marrow of the patients with IRP was positively correlated with the treatment effect of the disease (r=.764831, P <0.05).Conclusions:1. The generation of autoantibodies in the patients with Immune-related pancytopenia (IRP) is likely to have relationships with the total number of B lymphocytes and their cell subsets,CD+5B lymphocytes, in the bone marrow of the patients.2. There is a significant positive correlation between the percentage of B lymphocytes, accounting the bone marrow mononuclear cells (BMMNC) in bone marrow of the patients with immune-related whole pancytopenia (IRP),and the treatment onset time. Within a certain range, the higher the percentage, the slower the time of treatment onset.3. Flow cytometry(FACS) provides a reliable reference value for assisting in the diagnosis of immune-related whole pancytopenia (IRP).