The Research Of The Improvement Role Of Anemarrhena Water Decoction On The Rat With Chronic Emotional Stress By The Empty Bottles

Objective:To study rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress.Methods:The rats as the research object,make use of empty bottle stimulate cause the chronic emotional stress model,using the behavior observation and Enzyme league immune method, after chronic emotional stress, the Attack, exploration, behavioral modify and weight growth and the content of cortical ketones in the blood serum as the index to experiment.1、Through irrigating stomach0.54g/kg、1.08g/kg、2.16g/kg、4.32g/kg、8.64g/kg five different doses rhizoma anemarrhenae decoction, after giving the rats seven days,we determine all kinds of indexes, defining the dose-effect relationship for the rhizoma anemarrhenae improve chronic emotional stress.2、Through irrigating stomach for the five days, seven days, nine days, eleven days,fourth days, after the final irrigating stomach, we determine all kinds of indexes, defining the time-effect relationship for the rhizoma anemarrhenae improve chronic emotional stress.3、Based on the study of the dose-effect relationship, we make rats divide the blank group, model group,and intervention group.The blank group give two water, other time we don’t give water and empty bottle,making free of diet.Model group and intervention group based on the above provisions give empty bottle stimulus.every day give once and twice, which will continue14days,the intervention group will start to irrigate stomach after stimulating,the dose is2.16g/kg,the irrigation stomach will last seven days.After the final irrigation stomach we determine all kinds of indexes, To study rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress.4、Based on the experiment of the dose-effect relationship we make rats divide the blank group, model group,and treatment group. he blank group give two water, other time we don’t give water and empty bottle, making free of diet.Model group and treatment group based on the above provisions give empty bottle stimulus.every day give once and twice, which will continue14days, the intervention group will start to irrigate stomach after stimulating,the dose is2.16g/kg,the irrigation stomach will last seven days.After the final irrigation stomach we determine all kinds of indexes, To study rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress.Results:1、Based on the the study of the dose-effect relationship of the rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress.The rhizoma anemarrhenae decoction improve lever function empty bottle stimulate induced chronic emotional stress and the dose take on the certain correlation.making use of the content of cortical ketones in the blood serum as the index,in the five doses,the content of cortical ketones in the blood serum of the second dose(1.08g/kg) and the third dose (2.16g/kg) compared with the model group has the very significant difference(p<0.01),If we go on increasing the dose,the efficacy is last decreased.the attack behavior of the third dose group and fourth dose group compared with model group has significant decreased,and has the very significant difference(p<0.01). If we go on increasing the dose,the efficacy is last decreased.The behavioral modify of the second dose and the third dose has striking higher than model group,at the same time it has significant differences(p<0.01), If we go on increasing the dose,the efficacy is last decreased.2、Based on the the study of the time-effect relationship of the rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress.The rhizoma anemarrhenae decoction improve lever function empty bottle stimulate induced chronic emotional stress and the dose take on the certain correlation.making use of the content of cortical ketones in the blood serum as the index,in the five doses,the content of cortical ketones in the blood serum of the seventh day compared with the model group has the very significant difference(p<0.01),If we go on increasing the dose,the efficacy is last decreased. compared with,the seventh-day group and ninth-day group, the attack behavior has significant decreased,and has the very significant difference(p<0.01). If we go on increasing the dose,the efficacy is last decreased; Compared with the behavioral modify and the seven-day group the modify behavior has striking increased and it has significant differences(p<0.01), If we go on increasing the dose,the efficacy is last decreased.3、The study of the rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress for the intervention.Compared with intervention group and model group:attack behavior has striking decreased,and it has significant differences(p<0.01); the modify behavior has significant increased,and it has differences(p<0.05).Compared with the model group、intervention group and blank group,in the seven days in the stimulus,the weight growth has significant decreased,and it has differences (p<0.05),in the fourth days in the stimulus,compared with model group has differences (p<0.05).Compared with model group the content of cortical ketones in the blood serum of the intervention group has significant decreased,and it has significant differences (p<0.01).4、The study of the rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress for the treatment. Compared with the treatment group and the model group:the content of cortical ketones in the blood serum has striking increased,and it has significant differences(p<0.01); attack behavior and exploration behavior have significant increased,and it has differences(p<0.05); the weigh growth has striking decreased,and it has difference(p<0.05).If we stop stimulus,treatment group and model group the content of cortical ketones in the blood serum have decreased,treatment group has significant difference (p<0.05); compared with treatment group and model group:modify behavior has significant increased,and it has differences (p<0.05); the weight growth has significant increased,and it has differences (p<0.05). Conclusion:1.The best dose of the rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic psychological stress is the third dose(2.16g/kg),the best time is seven days.2.The rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress for the intervention.role.3.The rhizoma anemarrhenae for the improve lever function empty bottle stimulate induced chronic emotional stress for the treatment role.