Suppressors Of Cytokine Signaling Proteins-3Expression In Dcciduas And Villus Tissues Of Recurrent Spontaneous Abortion

Objective: Recurrent spontaneous abortion is defined as two or more prior spontaneousabortions. The pathogenesis is very complex, including gene, endocrine, immune,anatomy, infection etc. The incidence is rising. Immunomodulation research hasbecome a hot spot.The differentiation of helper T cell affects the normal pregnancy inmaternal interface. The dominant Th2type cytokines, have a positive role in thedevelopment of implantation embryos, Th1type cytokines from the immune-enhancingreaction, semi-foreign body antigen have an effect on embryo rejection. Cytokinesregulate immune responses through the combining with the receptor on the cell membrane, along the signal transduction pathway transcriptional expression ofbiologically active proteins,. Negative regulator of cytokine signal transduction can beinduced by cytokines, cytokine signal transduction plays a negative feedback regulationof protein molecules and reduce immune injury. To investigate the cytokine signaltransduction negative regulatory factor3(SOCS3) in deciduas and villus tissues ofrecurrent spontaneous abortion and normal pregnancy, maintaining the role ofmaternal-fetal immune tolerance interface.Methods: A total collection villus and deciduas of early pregnancy of18patients withrecurrent spontaneous abortion(test group), and that of22normal pregnancies as controlgroup in the same period. The expression of SOCS-3mRNA was evaluated by reversetranscriptase-polymerase chain reaction(RT-PCR), their protein expression byimmunohistochemistry streptavidin biotin-peroxidase (SP) in villus and deciduasrespectively and the position of SOCS3expression.Results: Between the two groups of general data analysis, the average age of the testgroup was (28.91±3.74) years old, the average gestational age was (8.75±1.40)weeks; control group average age was (30.15±3.21) years old, mean gestational agewas (8.38±1.10) weeks, comparing two groups of age and gestational age, thedifference was not statistically significant (P>0.05).Both groups expressed SOCS3:(1)RT-PCR analysis showed the average level of SOCS3mRNA expression in studygroup villi tissue is (0.34±0.09), SOCS3mRNA average expression level in studygroup decidual tissue is (0.38±0.08), SOCS3mRNA average expression level incontrol group villi tissue is (0.66±0.10), SOCS3mRNA average expression level incontrol group decidual tissue is (0.69±0.14), The level of SOCS3mRNA expression instudy group villi and decidua tissue were lower than that in the control group, thedifference was statistically significant (P <0.05); (2) Immunohistochemistry analysis of SOCS3expression in the cytoplasm of villoussyncytiotrophoblast cells and cell trophoblastic and decidual tissue stromal cells anddecidual cells cytoplasm.It showed that the average expression levels of SOCS3proteinin study group villi tissue is (1.22±0.43), the expression of SOCS3protein in decidualtissue is (1.50±0.51), the average of SOCS3protein expression levels in control groupvilli tissue is (2.72±0.46), the average SOCS3protein expression levels in deciduatissue is (2.82±0.39), comparing SOCS3protein in villi and decidual tissues of twogroups, the expression level of test group was lower than that in control group, thedifference was statistically significant (P <0.05).Conclusions:①The expression of SOCS3was low in recurrent spontaneous abortiontissues, which showed that SOCS3involved in the maintenance of normalmaternal-fetal immune tolerance interface.②The decreased expression may be one of important reasons to lead to recurrentabortion. The specific mechanism needs further study.