The Experimental Study On The Effect In Different Zones Of The Target Area After Radiofrequency Ablation And Clinical Observation Of Iiradiofrequency Ablation Combined With¹³¹I-chTNT In The Treatment For Pulmonary Malignant Tumor.

Objective：To analyze the differences in the effect of ablation in different zones of thetarget area after radiofrequency ablation.Methods：Thirty-six pulmonary tissues of pig lungs were divided into six differentgroups according to different ablation time. During the ablation procedure, recorded thetemperature change situation of the zone of ablation center, the needle tip zone of thecentral needle of RF needle, the needle tip zone of edge needle, the middle zone on thestraight line between central needle tip and the edge needle tip. After ablation, sampleswere collected from four different zones of the target ablation areas and the comparisonzones，dyed by hematoxylin and eosin and stained by Annexin V-FITC/PI, flowcytometry（FCM）was used to determine the apoptosis. Calculated the corrected celldeath rate of different zones in different ablation time.Results：The corrected cell death rate of ablation zone was higher than the one ofcomparison zone in every group（P＜0.05）.Before the cells were disappearedcompletely, the corrected cell death rate of the ablation center was higher than the oneof the other three ablation edge zone（sP＜0.05）. Ablation group in shorter time（s5min、10min、15min），the corrected cell death rates of the needle tip zone of RF needle ablation center and the needle tip zone of edge needle tip zone were higher than that ofthe middle zone on the straight line between Central needle tip and the edge needle tip（P＜0.05）.When the ablation time achieved20min,there was no difference in theeffect of ablation among the three ablation edge zones（P＞0.05）. Before the cells weredisappeared completely, the corrected cell death rate of ablation zones would increasealong with time extends. When the ablation time achieved30min, the cells in fourablation zones may disappear completely（P＞0.05）.Conclusion：Before the cells disappear completely, the ablation effect of the ablationcenter is better ablation edge zones. In shorter ablation times, there are differencesamong different ablation edge zones, but these differences will disappear if the ablationtime extended adequately. All target zones could disappeare completely if given enoughablation time. Objective: Radiofrequency ablation is a minimally invasive local technique used totreat pulmonary malignant tumor. Iodine-131-labeled chimeric tumor necrosis treatment(¹³¹I-chTNT) is a new immune therapy drugs targeted radiation. This study observes theclinical efficacy and safety of¹³¹I-chTNT radioimmunotherapy combined withradiofrequency ablation in the treatment for pulmonary malignant tumor.Methods:23lesions of23patients with pulmonary malignant tumor were treated bypercutaneous radiofrequency ablation combined with Local injection of¹³¹I-chTNT. Alllesions were evaluated by CT scan and surial，complications， hematology toxicity toevaluation therapeutic value and security of this treatment. Results: All23patients are successfully completed treatment. Two days aftertreatment，¹³¹I-chTNT could be found with tumor lesions in whole bodyradioimmunoimaging in all patients. One month after treatment， the white blood cellscount was decreased slightly compared with pre-treatment， but there was nosignificant different （t=0.645，p=0.525）.While the blood platelet count decreasedsignificantly compared with pre-treatment（t=4.436，p=0.000）. Three months aftertreatment， chest CT showed5cases in CR，7in PR，7in SD and4in PD. Theresponse rate （CR+PR） was52.17%（12/23），disease control rate（CR+PR+SD） was82.61%（19/23）. Six months after treatment， chest CT showed6cases in CR，5inPR，6in SD and6in PD. The response rate （CR+PR） was47.83%（11/23），diseasecontrol rate（CR+PR+SD） was73.91%（17/23）. Intraoperative did not happen crisis oflife serious complications，and1year survival rate was73.91%（17/23）;2years ofsurvival rate was45.45%（10/22）.Conclusion:¹³¹I-chTNT radioimmunotherapy combined with radiofrequency ablationis effective and safe in treating pulmonary malignant tumor with little adverse effectsand side effects.We suggested this treatment shall be further investigated for clinicalapplication.