The Expression And Significance Of CCL18in The Serum And Tumor Tissue Of Patients With Oral Squamous Cell Carcinoma

Oral squamous cell carcinoma (OSCC) is the most frequent malignancy in maxillofacial region.Even after the combined and sequential therapy, the5-year survival rates of OSCC patients are still ranging from50%-70%. Hence, it is quite beneficial for increasing the patients’survival rate and quality of life by further exploring the mechanism of OSCC initiation and development, which could, of course, facilitate finding more effective therapies. Researches have shown that the microenvironment that tumor cells depend on, especially various immune molecules, inflammatory factors and their receptors,is of vital importance in the initiation and development of tumors. CCL18is a newly discovered inflammatory chemokine. There have been researched relating CCL18with the pathogenic process of breast cancer, ovarian cancer, gastric cancer and so on.However,the expression and significance of CCL18in OSCC is still lacking. In our study, We employed ELISA and immunohistochemistry techniques to carry out the preliminary research on the expression of CCL18in the serum and tumor tissue of patients with oral squamous cell carcinoma.PurposeTo detect the expression of CCL18in the serum and tumor tissue of patients with oral squamous cell carcinoma and analyze its relations to clinical pathological parameters for the purpose of discovering the role of CCL18in the initiation and development of OSCC.Material and Method1.The expression of CCL18in the serum of patients with OSCC1.1Samples:the serum of59OSCC patients and17healthy adults;1.2Experiment:ELISA;1.3Analysis:nonparametric Mann-Whitney C/test.2.The expression of CCL18in the tumor tissue of patients with OSCC1.1Samples:97tumor tissues of OSCC,92adjacent non-cancerous tissues of OSCC,20normal oral mucosa;1.2Experiment:immunohistochemistry;1.3Analysis:nonparametric Mann-Whitney U test.Results1.The content of CCL18is higher in the serum of OSCC patients than that of healthy adults, and higher in late OSCC patients(phase III and IV) than that of early ones(phase land II), which are all statistically significant (P<0.05).2.The expression of CCL18is higher in the tumor tissues that those of adjacent non-cancerous and normal mucosa, higher in late OSCC patients(phase III and IV) than that of early ones(phase I and II), higher in patients with cervical lymph node metastasis than that of patients without, which are all statistically significant (P<0.05).ConclusionCCL18is abnormally expressed in the serum and tumor tissue of patients with OSCC, which is closely related to the clinical phase and cervical lymph node metastasis, suggesting CCL18may be of certain importance in the initiation and development of OSCC.