An Epidemiologic Study On Levels And Determinants Of Blood Glucose In Reproductive Women

Objective:To describe the distribution and explore the determinants of fasting blood glucose in pre-conception women, to provide evidence for prevention and control of gestational diabetes mellitus and other related diseases.Methods:1. With the cluster sampling, we randomly chosen9towns in37from Liuyang rural areas and recruited voluntary participants who plan to pregnancy in family planning center and pre-marriage clinics and consent to our study from Apr1,2008to Apr30,2011. We used χ2test for the single-factor analysis, and used the unconditional logistic regression for the multi-factor analysis.2. Collecting and determination of the blood samples:In the strict aseptic operation conditions, collected2tubes of peripheral blood (ordinary tube and anticoagulant tube) in subjects of fasting condition in the early morning,5ml each pipe, centrifugal separated serum and plasma and saved in-70℃refrigerator. Used Changchun Dirui CS-300T automatic biochemical analyzer for detection:blood glucose (Glucose oxidase method), triglyceride (GPO-PAP), cholesterol (CHOD-PAP), high density lipoprotein (PTA-Mg2+), low density lipoprotein (PVS precipitation method combined with Friedewald formula and used Friedewald formula, namely:LDL-C=TC-HDL-C-TG/2.2(mmol/L)), homocysteine (cycle enzymes method). Serum insulin, folic acid and cortisol were measured using radioimmunoassay method.3. Blood glucose diagnostic criteria:Formulated According to 《2010American Diabetes Association Guidelines for Standards of Medical Care of Diabetes》. low blood glucose:Fasting plasma glucose<3.6mmol/L, the normal blood glucose:3.6-5.5mmol/L, impaired fasting glucose:5.6-6.9mmol/L, hyper blood glucose:≥7.0mmol/L. In this article, we merged impaired fasting glucose group and the hyper glucose group into abnormally hyper blood glucose group for analysis.4. Survey included general condition (age, occupation, per capita household income, education), smoking (active smoking, passive smoking), previous pregnancy, et al. Physical examination included the measurements of blood pressure, height, weight and waist.Results:1. General condition:1386pre-conception women were investigated in our study. Age between20with43, education level between0to18years, mean10.49+2.27years; junior high school (56%) and agriculture (36.4%) is the most part of education and occupation, respectively, followed by the housework.2. The mean of GLU was4.76±1.14mmol/L, The prevalence of normal blood glucose was84.5%, low blood glucose was9.9%, impaired fasting glucose was3.7%, and the hyper blood glucose was1.9%.3. Multinomial Logistic regression analysis showed that higher serum cholesterol level (OR=2.42), higher triglyceride level (OR=3.28) and the number of pregnancy (OR=1.41) were associated with high-normal blood glucose.4. The results of correlation analysis showed that Pearson correlation coefficients between fasting blood glucose and cholesterol, triglyceride, low density lipoprotein were0.175,0.142, and0.179respectively.Conclusion:1. The prevalence of high-normal blood glucose was1.9%, impaired fasting glucose was3.7%in pre-pregnancy women, which was higher than the national level of female. 2.The serum cholesterol, triglyceride, the number of pregnancy, and low density lipoprotein level were associated with high-normal blood glucose.