Prelininary Study Of The Unstability Factors Of Atherosclerosis Plaques In Human Atherosclemtic With Type2Diabetes

Objective：AGE（advanced glycation end products，AGE）play a key role in diabetic vascular complications,whereas MMPs（matrixmetalloproteinases）contribute to plaque instability.This study was conductedto investigate the differences expression of AGE，RAGE，ECM and MMP-2inthe extent of atherosclerotic plaque specimens of lower extremity arteries ofpatients with or without type2diabeties,explore its affiliates.Throughdetection the proportions of MMP-2expression， CD68+macrophagesexpression and apoptosis in intimal areas association with RAGE expression，To investigate the mechanisms underlying atherosclerotic plaque stabilizationinduced by RAGE. Methods：This study based on deriving atherosclerosplaque specimens from lower limb arteries of patients with or without type2diabetes (n=8,each group).The morphology detection and initialhistopathologic changes of atherosclerotic plaques were observed byhematoxvlinarld eosin (HE) staining，and the expression of AGE，RAGE，MMP-2,and CD68+macrophages in the atherosclerotic plaques were observedby immunochemistry technology(SABC method).the expression of ECM wasdetected by Masson staining.Apoptosis of cell was determined by TUNELstaining. Results：The proportion of AGE accumulation,MMP-2expression in areas of atherosclerotic plaques of diabetics were greater than in nondiabetics.RAGE expression was significantly increased in the human atheroscleroticplaques of diabetics compared to nondiabetes,The difference wassignificant(P＜0.05).RAGE and MMP-2expression were linearly positivecorrelated with AGE accumulation and negatively correlated with theexpression of ECM. Plasma levels of HbA1c were linearly positive correlatedwith AGE accumulation, the expression of MMP-2, the expression of RAGEand apoptosis in intimal areas. Over-expression areas of RAGE compared tothe region of the relatively low-expression areas have more plaque instabilitycharacteristics: larger plaque, lipid necrosis greater accounting for plaquearea,significant increase in CD68+macrophages positive expression andTUNEL-positive cells (apoptotic cells) in Over-expression areas ofRAGE.Conclusions： The present study shows significant increases inAGE/RAGE and related MMP-2expression level in lower limb arteriesspecimens from the diabetic compare with nondiabetic specimens. and thatRAGE expression in diabetic plaques may be partially led to plaque instabilityand thus related to AGE/RAGE-dependent diabetic vascular complications.