| Objective:This study is to investigate the role ofCD4~+CD25~+regulatory T cells and transcription factor FoxP3on thepathogenesis of Infectious mononucleosis(IM) in children, and observe theexpression of TLR2(Toll-like receptors, TLRs) and TLR9on peripheral bloodmononuclear cells and the levels of plasma IL-2, IL-10, TGF-beta1, andIFN-gamma in children with IM. The experimental results may provide thetheoretical evidence of immunotherapy and new way for IM. Methods:1.Theexpression of TLR2,TLR9and FoxP3mRNA were detected by Real Time PCRusing SYBR Green I.2. In the plasma the expression of cytokine IL-2, IL-10and IFN-γ was detected by Luminex-100and the expression of TGF-β1wasanalyzed by ABC-ELISA.3. The expression of T lymphocyte subsets CD3~+,CD3~+CD4~+, CD3~+CD8~+and CD4~+CD25~+was detected by flow cytometry onperipheral blood mononuclear cells.4. The experiment data were analyzed bystatistical software SPSS16.0(one-way ANOVA). Results:1. The relative levelof TLR2mRNA in peripheral blood mononuclear cells of patients with IMacute stage group(4.03±0.56)was significantly higher than those of the controlgroup(2.22±0.57)(P＜0.01). The relative level of TLR2mRNA in peripheralblood mononuclear cells of patients with IM recovery group(2.76±0.83)was significantly lower than those of IM acute stage group(4.03±0.56)(P＜0.01).2.The relative level of TLR9mRNA in peripheral blood mononuclear cells ofpatients with IM acute stage group(8.88±1.56)was significantly higher thanthose of the control group(3.63±1.30)(P＜0.01). The relative level ofTLR9mRNA in peripheral blood mononuclear cells of patients with IMrecovery group (5.34±1.60) was significantly lower than those of IM acutestage group(8.88±1.56)(P＜0.01).3.The relative level of FoxP3mRNA inperipheral blood mononuclear cells of patients with IM acute stage group(2.82±0.90) lower than those of the control group (4.65±1.23)(P＜0.01).Therewas no significant difference between IM recovery group and the controlgroup(P＞0.05).4. The expression of plasma cytokinesIL-2, IL-10in IM acutestage group was42.97±16.36pg/ml,39.84±3.95pg/ml respectively. Theexpression of plasma cytokines IL-2, IL-10in control group was84.02±28.67pg/ml,69.86±3.95pg/ml respectively. The expression of plasmacytokines IL-2, IL-10in IM recovery group was58.85±19.42pg/ml,53.34±5.21pg/ml respectively.The expression of IM acute stage group waslower than those of the control group(P＜0.01).The expression of IM recoverygroup was higher than those of IM acute stage group.The expression of plasmacytokines IFN-γ(108.82±14.34pg/ml) and TGF-β1(488.15±117.5pg/ml)in IMacute stage group was higher than those of the control group (22.00±9.74pg/ml,253.75±72.6pg/ml) significantly (P＜0.01). The expression of plasmacytokines IFN-γ (49.82±11.55pg/ml)and TGF-β1(318.62±90.1pg/ml)in IM recovery group was lower than those of IM in acute stage group significantly(P＜0.01).5. The results of CD3~+, CD3~+CD4~+, CD3~+CD8~+, CD4~+/CD8~+,CD4~+CD25~+on peripheral blood mononuclear cells of IM acute stage groupwere (80.66±4.88%),(16.53±5.55%),(62.71±8.76%),(0.26±0.12),(2.38±1.32%)respectively.The results of control group were(63.04±5.51%),(37.98±4.20%),(27.71±6.52%),(1.37±0.45),(7.85±1.97%)respectively. There was significant difference between IM acute stage groupand the control group (P＜0.01).The results of CD3~+, CD3~+CD4~+, CD3~+CD8~+,CD4~+/CD8~+on peripheral blood mononuclear cells of IM recovery groupwere(74.25±7.33%),(29.89±6.03%),(42.25±7.33%),(0.71±0.19) respectively.There was significant difference between IM acute stage group and IMrecovery group (P＜0.01).The results of CD4~+CD25~+on peripheral bloodmononuclear cells of IM recovery group was (6.81±1.84%).There was nosignificant difference between the control group and IM recovery group (P＞0.05).6.CD4~+CD25~+Treg and expression of FoxP3mRNA is positivecorrelation and the correlation coefficient is0.823(P＜0.05).Conclusion:1.There is functional disorder of T lymphocyte subsets at acute stage of IM inchildren. The expression of CD4~+CD25~+regulatory T cells was reduced, and itsspecial transcription factor FoxP3mRNA was decreased, which led to theinsufficient immunosuppressive effects that may be one of the importantreasons of the immune imbalance. Therefore, the results provided experimentalevidence for illustrating the pathogenesis of IM from regulatory T cells and its molecular mechanism regulation.2.This study proved that pathogenesis of IMinvolved in the TLR2and TLR9.3. IFN-γ, IL-2, TGF-beta1and IL-10wereproved to involve in pathogenesis of IM. |
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