| Objective:We observe the damage of formaldehyde (FA) on liver function through the detection of serum total protein, albumin concentration and alanine aminotransferase (ALT/GPT) activity and liver tissue glutathione (GSH) and malondialdehyde(MDA) content, superoxide dismutase (SOD) activity of formaldehyde exposure mice. We use the concept found in the mouse liver damage lowest exposure concentration following an experimental concentration of formaldehyde exposure. At the same time give tea polyphenols (TP) gavage, and observe the protective effect of tea polyphenols on formaldehyde-induced liver damage. Provide a theoretical basis for the prevention of environmental hazards of formaldehyde contamination in the future by the tea daily way of life.Methods:We selected40healthy adult Kunming white mice. The mice were divided into three formaldehyde exposure groups (the exposure concentrations were40mg/m3,80mg/m3and160mg/m3) and a control group in accordance with the simple randomized. Every group has10mice, male and female each half. We use four volume of fifty liters glass exposure cylinder conducting static inhalation. Cylinder ventilate interval of two hours. The daily exposure time is eight hours, continuous exposure of twenty-eight days. Twenty-four hours after the end of the exposure, we use2%sodium pentobarbital anesthetized mice. We take the mixed blood using the method of enucleation and then separation of the liver tissue. We detected in the serum total protein, albumin concentration and ALT/GPT activity and liver tissue GSH and MDA, SOD activity with spectrophotometry. We analyzed formaldehyde damage to the liver function by comparing the differences of these indicators between the three exposed groups and the control group, and to identify the lowest concentration of formaldehyde can cause liver dysfunction in mice. Re-select fifty healthy adult Kunming white mice, divided into five groups according to simple random grouping method, a blank control, four groups of formaldehyde static inhalation,(the lowest concentration of formaldehyde can cause liver dysfunction in mice in the first part of the study as part of the formaldehyde exposure concentration, and one group only with formaldehyde exposure, the remaining three in formaldehyde exposure at the same time every day were given100mg/kg,200mg/kg,400mg/kg concentration of polyphenols gavage). Mice were daily exposed to formaldehyde for eight hours, and continuous exposure to twenty-eight days. Twenty-four hours after the end of the exposure, we detected in the serum total protein, albumin concentration and ALT/GPT activity and liver tissue GSH and MDA, SOD activity, and compare the differences between each of these indicators to analyze the protective effect of tea polyphenols on liver function damage. Results:The first part:Toxic effects in mice exposed to formaldehyde:1.1Exposed to formaldehyde concentrations of160mg/mj affect the body weight of mice growth compared with the control group were significantly different (F=7.032, P=0.001);1.2Formaldehyde exposure group liver index higher than that in the control group (F=12.074, P=0.000), and showed a dose-response relationship.1.3Exposed to formaldehyde concentration of80mg/m3and160mg/m3, serum total protein (F=13.014, p=0.000) and albumin levels (F=28.923, p=0.000) lower than the control group, and ALT/GPT vitality higher than that in the control group (F=2.514, p=0.045) and showed a dose-response relationship.1.4Formaldehyd-e exposure concentration of80mg/m and160mg/m, the GSH content in liver tissue (F=3.39, p=0.028) and SOD activity (F=12.767, p=0.000) lower than the control group, and MDA level was higher than the control group (F=12.792, p=0.000), and showed a dose-response relationship. The second part:The protective effect of tea polyphenols on formaldehyde-induced mouse liver damage:2.1Formaldehyde group body weight of mice is lower than the blank control group (F=2.622, p=0.003). Various tea polyphenols protect mice body weight and blank group was no significant difference (F=2.528, p=0.054);2.2The formaldehyde mice liver index is higher than control group (F=10.963, p=0.003). Tea polyphenols protect the mice liver index and the blank group was no significant difference (F=1.523, p=0.211);2.3Various tea polyphenols protect mice serum albumin concentration lower than blank control group, but higher than the formaldehyde group (F=20.09, p=0.000). The total protein content is lower than the blank control group, but higher than the formaldehyde group, and high concentrations of tea polyphenols were significantly lower than the formaldehyde group (F=14.138, p=0.037). ALT activity of various tea polyphenols protect the group and blank group was no significant difference (F=2.059, p=0.125), and high concentrations of polyphenols group lower than in the formaldehyde group (F=5.371, p=0.048);2.4GSH content in liver tissue of the tea polyphenols group (F=1.653, p=0.178) and SOD activity (F=0.713, p=0.55) with the control group comparison was not statistically significant. GSH content of polyphenols high concentration group (F=5.416, p=0.041) and SOD activity (F=5.243, p=0.022) higher than the formaldehyde group. Group of moderate concentrations of polyphenols and high concentrations of MDA content lower than the formaldehyde group (F=12.684, p=0.021). Conclusions: The formaldehyde concentration in the80mg/m3can lead to damage of the liver function, and there is a significant dose-effect relationship. Tea polyphenols on formaldehyde-induced liver injury in mice has a protective effect in a dose-effect relationship. Through eating or drinking water containing tea polyphenols rich foods or drinks reduce liver damage caused by the environmental formaldehyde pollution. |
PDF Full Text Request