Experimental Study On The Treatment Of Spontaneously Hypertensive Rats By Transcutaneous Electrical Nerve Stimulation

Bjective: Neurogenic hypertension is the major morbidity in the form of essentialhypertension. Single treatment of neurogenic hypertension, drug therapy is prone tofluctuations in blood pressure, and the need for lifelong medication. By transcutaneouselectrical nerve stimulation in the treatment of spontaneously hypertensive rats tostudy the signal transduction pathway and mechanism of neurovascular. Explore thetheoretical basis for the treatment of neurogenic hypertension, transcutaneouselectrical nerve stimulation and to find a new experimental theory and basis for thephysical treatment of hypertension.Methods: In this study, spontaneously hypertensive rats（SHR） as the object ofstudy,WKY rats were used as the comparative experiment. Appropriate electricalstimulation therapy parameter model, electrical stimulation of specific parameters:voltage20V, the stimulation frequency200Hz, pulse width <0.1ms stimulus waveformLilly waveform. We use non-invasive rat tail cuff arterial blood pressure monitoringrecords, including the dynamic changes of the arterial systolic blood pressure(SBP),arterial diastolic blood pressure(DBP), mean arterial blood pressure(MBP) and heartrate(HR). Using radioimmunoassay (RIA) detection of plasma angiotensin Ⅱconcentration changes. According to the principle of the immune mechanism ofcompetition, the angiotensin Ⅱ antibody iodine combination of matter and plasma angiotensin Ⅱ immune competition reactions, and immune separation agent willcombine part of the free part of the separation, determination of the binding portion ofthe radioactivity. Using direct ion-selective electrode assay before and after treatmentthe concentration of sodium in the blood circulation of rats, analysis of serum sodiumconcentration of the antihypertensive effect. The study of transcutaneous electricalnerve stimulation on changes in morphology and function of the SHR cardiovascularcenter, mitotic activity and protein synthesis observed solitary tract nucleus(NTS)neurons by hematoxylin eosin(HE) staining. Using radioimmunoassay imagingtechniques, analysis iodine optical density/area ratio(Iod/Area) of the125I-sarcosine1isoleucine8-Angiotensin Ⅱ recptor1(125I-SI-AngⅡ). the study of the solitary tractnucleus area of angiotensin Ⅱ receptor expression levels and changes in blood pressurebetween dialectical contact.Results: Transcutaneous electrical nerve stimulation group treatment ofspontaneously hypertensive rats(shrTENS) before and after treatment of the arterialblood pressure decreased significantly (P <0.05). ShrTENS with the feeding ofvalsartan spontaneous hypertensive rats(drug) compared the antihypertensive effect,no significant difference (P>0.05). Transcutaneous electrical nerve stimulationtreatment of the hypertensive effect than the drug group relaxation andstability;.Transcutaneous electrical nerve stimulation no significant blood pressurelowering effect on normal blood pressure in Wistar Tyoto rats group(wkyTENS),arterial blood pressure in WKY rats before treatment and after treatment (P>0.05), nosignificant difference. All experimental samples was not observed in the case of lowblood pressure. Each experimental group rat’s heart rate, body weight, serum sodium concentration before treatment and after treatment showed no significant difference (P<0.05). No direct contact with plasma angiotensin Ⅱ(AⅡ) concentration and arterialblood pressure, feeding valsartan in spontaneously hypertensive rats plasmaangiotensin Ⅱ concentrations were significantly increased before treatment and aftertreatment (P <0.05), there are significant differences. Analysis of shortens,control,wkyTENS before treatment and after treatment of angiotensin Ⅱ concentrationwas no significant difference (P>0.05). Concentration of angiotensin Ⅱ of SHR morethan in WKY angiotensin Ⅱ at concentrations (P <0.05) difference was statistically.Heterochromatin in neurons of the the comparison shrTENS the NTS cell countheterochromatin neurons of the NTS with drug counts, P>0.05, no significantdifference, Heterochromatin in neurons of the the comparison shrTENS the NTS cellcount heterochromatin neurons of the NTS with drug counts, P>0.05, no significantdifference, comparison shorTENS with control group and wkyTENS, P <0.05,difference significant. Analysis of angiotensin Ⅱ receptor1an RIA prompt blankself-developing Iod/Area ratio in the control group the iodine binding rate is thehighest. Comparison of shrTENS group and the drug control group no significantdifferences P>0.05. WkyTENS group iodine combined with the lowest rate, P <0.05,there are significant differences.In this study, treatment of spontaneously hypertensive rats by transcutaneouselectrical nerve stimulation, the treatment of spontaneously hypertensive rats fed withvalsartan antihypertensive effect, mainly in the systolic and diastolic blood pressuresignificantly decreased. Electrical stimulation treatment group and the control grouparterial blood pressure significantly different. Changes in the observed solitary tract nucleus neurons in cell morphology and function, and angiotensin Ⅱ receptorexpression in a radioimmunoassay autoradiography experiments confirmedtranscutaneous electrical nerve stimulation therapy can improve the cardiovascularcenter of the SHR, since steady staterestore normal levels of arterial blood pressure setpoint, regulation of autonomic nervous function, arterial blood pressure returned tonormal.