Preparation Of Gentamicin-loaded Electrospun Coating On Titanium Implants And A Study Of Their Properties In Vitro

Objective：In this preliminary study, gentamicin-loaded coating on titanium implantswas prepared using the electrospinning technique, and some properties of the coatingtitanium implants were studied. Implant-related infections are disastrouscomplications in the clinic, and there are no effective therapies. Local applicationof the antibiotics has attracted more attention to prevent and treat the implant-releatedinfections because of its high local drug level. So this experiment preparedgentamicin-loaded coating on titanium implants and studies its properties.Methods (1) Adopted an electrospinning technique to prepare a gentamicincoatingon the surface of a three-hole titianium implant.(2) The morphology of thegentamicin-coated titanium implants was observed using a Hitachi S4800scanningelectron microscope. The amount of the antibiotic, gentamicin, contained in thecoating and the in vitro release behavior of the gentamicin from the coated implantswere studied.(3) The antibacterial efficacy of and bacterial adhesion to thegentamicin-coated titanium implants in vitro was studied.(4) The mouse MC3T3-E1osteoblasts was used as the experimental cell and the cytotoxicity of thegentamicin-coated titanium implants on mouse MC3T3-E1osteoblasts wasinvestigated in vitro by CCK-8.Results (1) The morphology of the gentamicin-loaded coating exhibited nanofiberswith smooth surfaces and round cross sections by SEM. The mode diameter of thenanofibers was650-750nm (average of705±122nm) by Image J. The mean weight ofgentamicin was392.2±57.2μg for each gentamicin-coated titanium implant. The release behavior of gentamicin revealed an initial burst release during the first24h,followed by a slow and continuous release during subsequent weeks and at the end ofthe2-week test period,71%(approximately278.3lg) of gentamicin had beenreleased.(2) The gentamicin-coated titanium implants had a persistent antibacterialefficacy for1week and significantly reduced the adhesion of the Staphylococcusaureus compared with bare titanium implants in vitro(p<0.05).(3) The quantitativeresults were obtained using a CCK-8assay shows the gentamicin coating canpromote cell migration, adhesion and proliferation compared with the control groupand there have the statistical significance. The gentamicin coating has good cellcompatibility without cytotoxicity.Conclusion (1) The gentamicin-coated titanium implant was prepared byelectrospinning. This method is an effective way, which has a lot of advantages suchas time saving, cost-efficient and can be easily employed in laboratory and industrialsettings.(2) The gentamicin-coated titanium implant shows a persistent release ofgentamicin in vitro. And this kind of titanium implant can reduce the adhesion of theStaphylococcus aureus and inhibit Staphylococcus aureus growth in vitrosignificantly.(3) The gentamicin coating can promote cell migration, adhesion andproliferation because of its high-specific surface area and high porosity of thenanofibers, and the cytotoxicity was not observed.(4) The gentamicin-coatedtitanium implant which was prepared by electrospinning shows well properties invitro and can be study more in-depth in the field of prevention and cure theimplant-releated infection.