Nuclear Transcription Factor Kappa B Small Interfering RNA Expression Vector Construction And Screening

Objective: to design and build, screening for NF kappa B subunit RelA (p65) ofsmall interference RNA expression vector.Methods: the Invitrogen miRNA design system, the design and synthesis of oligos,through recombinant DNA technology, synthetic double-stranded miRNA oligo intoa miRNA expression vector pcDNA than6.2-GW/EmGFPmiR, and by sequencingfor identification, then through transient transfection technology will build miRNAexpression vector was transfected into target cells of Panc I cell, through quantitativePCR clear synthesis of miRNA expression plasmid on pancreatic cancer Panc I cellsin NF kappa B expression influence.Results: through the sequencing, identification of specific recombinant clone insertfragment sequences and design of the oligo sequence is completely consistent,interference vector construction is successful, PancI cell transfection efficiency ofabout50%, screening of effective miRNA expression plasmid.Conclusion: the successful design and construction, screening for NF kappa Bsubunit RelA (p65) of small interference RNA expression vector. To further studythe NF kappa B in pancreatic cancer cell proliferation and growth of foundation.