| Objective:Examined the effects of kidney side [Hospital preparations (the Hubei drug pharmaceutical word Z20113160)] make the impact of kidney essence virtual rat liver regeneration index and bone marrow cell proliferation, investigate the effect and its mechanism that the therapeutic of nourishing kidney to produce marrow and form liver treatment essence deficiency of liver and kidney rat, reveal scientific content and the syndromes of the essence of Chronic Hepatitis B Liver Kidney Yin Deficiency, clear the objective basis and quantitative evaluation of "chronic-illness into the kidney "or" Illness into the kidney", lay a solid experimental basis of bone marrow stem cell transplantation or Kidney Liver and bone marrow stem cell transplantation in the treatment of CHB of essence deficiency of liver and kidney, provide a scientific basis of treatment CHB of essence deficiency of liver and kidney.Methods:On the second day to tenth day, make five time injection L-monosodium glutamate-saline solution to the subcutaneous of neonatal wistar rat, which dose for each4mg/g, and injection medical saline to the subcutaneous of control rats, normal control group without any treatment. Wean after28days, randomly divided into normal control group, saline control group, sham operation group, model group and kidney group after the time of6week. The kidney rats given3g/kg of the kidney (the hospital preparation), and the remaining groups were given the same amount of normal saline. Continuous administration of two weeks, PH the resection of the left liver lobe and the mid liver lobe, sham operation group flip liver and no resection the liver. Postoperative continued administration. Analysis of liver regeneration degree of liver regeneration index and liver mitotic index MI changes, observed the count of peripheral red blood cell and the content of hemoglobin, analysis of bone marrow mononuclear cells CD34/CD45dual-positive rate of change, observe the CD34expression of liver tissue and the fibronectin FN of liver tissue and pituitary expression. Biopsy photo using Image Pro-Plus6.0software for image processing, selected analysis of regional, obtain the mean optical density value of MOD. All the data using statistical software SPSS19.5Chinese version handling,multiple samples between each groups were compared using multi-factor analysis of variance, according to the charting software statistical results.Results:Serious disorders of the essence deficiency of liver and kidney rats’Liver Regeneration, at the first postoperative day they performance liver regeneration hyperthyroidism, Liver regeneration degree (0.34±0.10) was significant ly higher than the PHx group (0.22±0.08) and saline control group (0.21±0.05), the regeneration index (1.88±0.14) is higher than the PHx group (1.46±0.13) and normal sal ine group (1.47±0.18), lower than the MSG-sham operation group (3.40±0.11), liver cell division index of the MI (1.05±0.12) was higher than the PHx group (0.86±0.16) and saline control group (0.87±0.16). Advanced liver regeneration process is being significantly inhibited, liver regeneration after3,7and10days respectively,0.42±0.08,0.57±0.12,0.70±0.08, lower than the PHx group and control group; The liver regeneration index were2.07±0.04,2.41±0.06,2.84±0.10, lower than the PHx group, saline control group and MSG-sham operation group; the MI were1.04±0.18,0.41±0.16,0.15±0.03, significantly lower than PHx group and the saline control group. In end model group were lower than other groups in the degree of liver regeneration, regeneration index and liver cells, mitotic index, and can not be restored to normal levels. After treatment by" nourishing kidney to produce marrow and form liver "of the hospital preparation (E drug, pharmaceutical word Z20113160), the rat model of liver regeneration chaotic situation must rectify. After PHx lday liver regeneration degree (0.13±0.07) compared with model group (0.34±0.10) and PHx group (0.22±0.08), liver regeneration index (1.52±0.11) was significantly lower than model group (1.88±0.14), liver mitotic index MI (0.77±0.14) compared with model group (1.05±0.12), prompt us that the rat model of liver regeneration in early abnormal hyperactivity was inhibited. After PHx7and10days of nourishing kidney to produce marrow and form liver into the liver group rats the degree of liver regeneration were0.50±0.05,0.74±0.06,0.86±0.03, compared with model group was significantly increased; liver regeneration index were2.19±0.11,2.84±0.12,3.27±0.06, higher than the model group; liver mi tot ic index were1.89±0.13,0.57±0.20,0.28±0.04, significantly higher than the model group. Peripheral blood red blood cell RBC count and hemoglobin Hb content results show that MSG-sham operation group was significantly lower than the saline group, model group was significantly lower than the MSG-sham operation group, and by the " nourishing kidney to produce marrow and form liver" treatment, kidney and marrow into the liver peripheral blood RBC count, return to normal of Hb content. Flow cy tome try analysis showed that postoperative1day,3days,7days,10days, CD34/CD45double positive cells rate in bone marrow cells of the model group rat less than PHx group and the saline control group,(the difference was significant, P<0.05). To give a " nourishing kidney to produce marrow and form liver" treatment, the group of nourishing kidney to produce marrow and form liver into the liver tissues of rat bone marrow CD34/CD45double positive cells was significantly increased in the postoperative three days, seven days,10days were significantly higher than the model group. The difference was significant (P<0.05). The expression of CD34of experimental rat1iver tand CD34/CD45double positive cells of bone marrow in the rate of change in trend after1day,3days7days10days, CD34of model liver tissue expression lower than the PHx group and physio logical the saline control group, the difference was significant (P<0.05). The model of essence deficiency of liver and kidney group liver tissue CD34expression was significantly increased after3days,7days,10days were significantly higher than model group, the difference was significant (P<0.05). The model of essence deficiency of liver and kidney group (model group) after3,7and10days of liver tissue FN average density value is higher than the PHx group and the saline control group, the difference was significant, P<0.05. The model of essence deficiency of liver and kidney group after surgery,7and10days in liver tissue FN average density value is lower than the model group, the difference was significant (P<0.05); FN,3days after the average density value is higher than model group. The difference was significant (P<0.05). Pituitary FN of model rats postoperative1,3,7and10days the average density value is higher than the PHx group and saline control group, the difference was significant (P<0.05). The model of essence deficiency of liver and kidney group into the pituitary FN average density value lower than the model group, the difference was significant (P<0.05); three days after FN average density values than the model group, differences in the liver group were1,7and10dayssignificant (P<0.05).Conclusion:The model of essence deficiency of liver and kidney (MSG-liver regeneration-rat), which liver regeneration process disorder, manifested as early hyperthyroidism, then inhibition them, in end they can not be restored to a normal level of liver regeneration. Improved to some extent by nourishing kidney to produce marrow and form liver (E medicine pharmaceutical word Z20113160)treatment, after treatment of the rat model of liver and kidney essence deficiency, liver regeneration chaotic situation to a certain degree to correct." nourishing kidney to produce marrow and form liver regeneration mechanism may lie in the regulation of proliferation of bone marrow stem cell or migration to the damaged liver participate in liver regeneration and repair, as well as through the hypothalamus-pituitary-liver axis improvement intrahepat ic regenera t ionrepair micro-environment. |
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