Study On The Tachorevascularization Of Tissue Engineering Bone Improved By ASCs

Objective: To evaluate the efficacy of bone repair of rapid production of tissueengineering bone based on combining adipose-derived stem cells and bone marrowmesenchymal stem cells implanted rabbit posterior lumbar interbody fusion model, sinceadipose-derived stem cells may promote angiogenesis and bone marrow mesenchymalstem cells have good osteogenesis. Methods: The75male New Zealand rabbits wererandomly divided into5groups. These groups were implanted respectively in the lumbar5,6intertransverse with: Group A: BMSCs/allograft iliac bone with ASCs, GroupB:BMSCs/allograft iliac bone with physiological saline, Group C: allograft iliac bonewith ASCs, Group D: allograft iliac bone with physiological saline, Group E: autologousiliac bone. Each group was detected by PET/CT to contrast the standard uptake value in1,3,5weeks postoperative. Results: PET/CT images of all groups grafting showedvarying degrees of bone fusion and bone metabolism enhancement. At the5th week, CTimages showed that there were bone callus in the adipose-derived stem cells/bonemarrow mesenchymal stem cell/allogeneic bone group, bone marrow mesenchymalstem cell/allogeneic bone group and autologous bone group, and residual cortical bonestrips out of district. It is uneven texture of bone graft district and more cortical bonearticle residuesin the adipose-derived stem cells/allogeneic bone group and allogeneicbone group. PET and of PET/of CT fusion images showed that there were uniformlydistributed strip concentration in the adipose-derived stem cells/bone marrowmesenchymal stem cell/allogeneic bone group, bone marrow mesenchymal stem cell/allogeneic bone group, adipose-derived stem cells/allogeneic bone group and autologousbone group, however, there were radioactive concentration at the both ends of bone graftdistrict, but not in the middle region in the allogeneic bone group. Conclusion: It wasgood efficacy of osteogenesis and vascularization for rapid production of tissueengineering bone based on combining ASCs and BMSCs.