Effect Of The Decompression Of Optic Canal Surgery And Dexamethasone To The TNF-α Immune Activity In Retinal Ganglion Cells After Opotic Nerve Injury

Objective: to investigate the effect of tumor necrosis factor-α to the opotic nerveand retinal ganglion cells after acute injury and decompression of opotic canal via pterionapproach or dexamethasone;survival RGCs after acute injury and decompression ofopotic canal via pterion approach or dexamethasone. Method:96New Zealand rabbitswere all setted up optic nerve injury models with right eye and were divided randomlyinto3groups，Surgery group,Dexamethasone group and injury group,24rabbits pergroup.Every group was further divided into4groups by the injury time(3d,7d,14d,21d).The Surgery group（24models）was treated with decompression ofopotic canal via pterion approach during24h-48h after injury models established;Dexamethasone group was treated with the continuing dexamethasone injected(1mg/kgonce per day).All models of3groups were killed and taken tissues at differenttime.Tissues were dyed with H&E staining and counted in each section using lightmicroscopy and detecting concentration of TNF-α using ELISA. Result:(1)The numbersof RGCs in Injury group were significantly missing.The numbers of survial RGCs inInjury group were significantly less than Surgery group’s and Dexamethasone group’s.(2)Dexamethasone group’s survial RGCs were less Surgery group’s in3day and7day,it hasstatistically significant; Surgery group’s urvial RGCs were little higher thanDexamethasone group’s,but it has no statistically significant.(3)The concentration ofTNF-α in Surgery group and Dexamethasone group was significantly lower than Injurygorup in different time.(4) The concentration of TNF-α in Surgery group was less thanDexamethasone group with statistically significant in3d,7d,14d;it has no statisticallysignificant between Surgery group and Dexamethasone group in21d. Conclusion: Ourdata suggests that a progressive decline in the number of viable RGC counted at3d,7d,14d,21d after acute opotic nerve injury;the observation that treatment withdecompression of opotic canal via pterion approach during24h-48h or withdexamethasone after injury models established prevents the RGC loss.Meanwhile,decompression of opotic canal via pterion approach and dexamethasone can inhabit theacute rising concentration of TNF-α.