Analysis Of The Differentially Expression Long Non-coding RNAs Profiles In HBV-Related Hepatocellular Carcinoma

Objective To investigate the disordered expression long non-codingRNAs(lncRNAs) profiles in hepatocellular carcinoma (HCC) tissues(Group T) andcorresponding adjacent non-tumoral liver tissues (Group N) from Non-HBV-RalatedHCC (Type1) and HBV-Ralated HCC (Type2). Methods Total RNA wasextracted from each frozen stored tissue. RNAs were checked its quality and integrity.And then the RNA was reverse transcribed into double strand cDNA. After the cDNAwas transcribed into biotinylated cRNA target，purified and fragmented it according tothe manufacturer’s instructions. Utilize lncRNAs hybridization-based microarraystechnology to detemine the disordered lncRNAs expression profiles between humanHCC and corresponding adjacent non-tumoral tissues, screen out the significantlydisordered expression lncRNAs after pretreatment and homogenization of raw data,and make unsupervised hierarchical clustering and volcano scatter diagram analysis.Results To identify the differentially expressed lncRNAs, we performed a FoldChange and T-test screening between the two groups obtained from the experiment.The threshold we used to screen Up or Down regulated lncRNAs is FoldChange>=1.5and P-value<=0.05. By Comparing differentially expressed lncRNAslevel of Group N1with Group N2,553lncRNAs disordered,285up-regulated, while268deregulated by more than1.5times, and127disordered by more than2times,78up-regulated, while49deregulated. Group T1vs Group N1,1427lncRNAsdisordered,752increased, while675deregulated by more than1.5times, and684disordered by more than2times,362up-regulated, while322deregulated. Group T1vs Group T2,423lncRNAs disordered,253up-regulated, while170deregulated bymore than1.5times, and82disordered by more than2times,39up-regulated, while43deregulated. Group T2vs Group N2,2700lncRNAs disordered,1275up-regulated,while1425deregulated by more than1.5times, and1536disordered by more than2times,741up-regulated, while795deregulated. Conclusions The disorderedexpression lncRNAs profile of HBV-Ralated hepatocellular carcinoma changessignificantly in comparison with corresponding adjacent non-tumoral liver tissues.The significant disordered expression lncRNAs may include a fair proportion ofcancer promoting lncRNAs and cancer-inhibiting lncRNAs, taking the critical role ofregulatory molecular mechanisms in HBV-Ralated hepatocellular carcinoma．...