Effects Of Sulphur Dioxide On Glycolipid Metabolism

Chapter1:A large number of epidemiologic studies have found associations between SO2and daily mortality, hospital admissions, and morbidity among persons with diabetes and cardiovascular disease. Therefore, the effect of SO2on diabetes and cardiovascular disease that has drawn widespread attention of the society. Many scholars have done a lot of research in epidemiology. But the mechanism of research was less that did not meet for treatment and prevention needs of diabetes and cardiovascular disease. We uses various biochemical technology researched long-term exposured to low concentrations of SO2on related gene expression of mRNA, biochemical indexes and FASN enzyme activity in rats.Chapter2:It was researched the effect of SO2(0and3.5,7.0,14.0mg/m3) on body weight, blood glucose, free fatty acid, triglyceride, insulin, liver glycogen and muscle glycogen and low density cholesterol in rats by using a variety of biochemical method and apparatus. The results were showed body weight of rats increased significantly, serum insulin secretion was significantly reduced, blood glucose levels are significantly elevated, glycogen synthesis first decreased then increased, pancreatic beta cell function even in low concentrations could significantly reduce, free fatty acids and content of triglyceride increased, low density lipoprotein cholesterol significantly decreased, high density lipoprotein cholesterol/total cholesterol ratio increased. SO2was an important virulence factor in diabetes mellitus.Chapter3:In order to study the molecular mechanism of occurrence of diabetes andcardiovascular disease exposure to SO2, the mRNA levels of FASN, SREBP, PPAR, FABP, PEPCK, GCK, GYS and ACC were analyzed in livers of rats after exposed to different concentrations of SO2(0,3.5,7.0,14.0mg/m3,4h/day, for30days) by using a real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) assay. These results lead to a conclusion that exposure to SO2could change the expression of diabetes-related genes. It indicated that it may be associated with inflammation and insulin secretion.Chapter4:The effects of long-term exposure to low concentrations of SO2on fatty acid metabolism in rats. It is discussed association between the FASN gene expression levels of mRNA and its enzyme activity. It suggested that enzyme activity was increased. The results indicated that it may be the effects of insulin and glucose.