Expression Of IL-8and RANTES In The Mouse Models Following Exposure To2,3,7,8-Tetrachlorodibenzo-p-Dioxin(TCDD)

Objective:To study the expression and significance of interleukin-8(IL-8) and regulated on activation,normal T cell expressed and secreted (RANTES) in peritoneal fluids, eutopic endometrium and ectopic emdometriu-m of the mouse models following exposure to TCDD.Methods:Selecting60pregnant specific pathogen free (SPF) C57BL/6mice.Their female offspring received TCDD(0,3or lOug/kg) in different time. The offspring were divided into five group(pre/postnatal):0/0、0/3、3/0、3/3、3/10.The endometriosis mouse model was established with autotransplantation of endometrium. Surgery to induce endometriosis in mice was performed at the seventieth day after birth. During week12postsurgery, animals were euthanized; peritoneal fluids, eutopic endometrium and ectopic endometrium were collected. The levels of IL-8and RANTES in peritoneal fluids were dete-rmined by ELISA. The expression of IL-8and RANTES in eutopic endometri-um and ectopic endometrium were measured with immunohistochemistry.All the data were analyzed with SPSS13.0statistical software.Results wer-e shown with x±s, applied with analysis of multi-mean variance (ANOVA) or Pearson χ2. There was significant difference on statistics if the value of P was less than0.05.Results:1There was no significant differences of general survival rate of explants between the TCDD exposure group and the control group; within each group the survival rate of explants which were transplanted into different parts of mice had difference.2The levels of IL-8in peritoneal fluid in mice were higher in the TCDD exposure group (0/3、3/0、3/3、3/10) than those in control group (0/0)(235.5116±21.0096,229.5184±18.6326,255.0822±22.3552,288.9307 ±28.9095,209.9283±9.2672, pg/ml)(p<0.05); in the group of the mice which received TCDD both in the prenatal and postnatal period, the levels of IL-8were higher in the high-dose exposure group (3/10) than those in the low-dose exposure group (3/3)(p<0.05); the levels of IL-8in the mice which received TCDD only in the prenatal period(3/0) were higher than those in control group (0/0)(p<0.05).3The levels of RANTES in peritoneal fluid in mice were higher in the TCDD exposure group (0/3、3/0、3/3、3/10) than those in control group (0/0)(0.9371±0.1351,0.9649±0.0744,1.1383±0.0919,1.3191±0.1356,0.8238±0.0832, ng/ml)(p<0.05); in the group of the mice which received TCDD both in the prenatal and postnatal period, the levels of RANTES were higher in the high-dose exposure group (3/10) than those in the low-dose exposure group (3/3)(p<0.05); the levels of RANTES in the mice which received TCDD only in the prenatal period(3/0) were higher than those in control group (0/0)(p<0.05).4The levels of IL-8in eutopic endometrium in mice were higher in the TCDD exposure group (0/3、3/0、3/3、3/10) than those in control group (0/0)(0.2851±0.0335,0.3041±0.0280,0.4081±0.0278,0.4624±0.0350,0.2202±0.0149)(p<0.05); in the group of the mice which received TCDD both in the prenatal and postnatal period, the levels of IL-8were higher in the high-dose exposure group (3/10) than those in the low-dose exposure group (3/3)(p<0.05); the levels of IL-8in the mice which received TCDD only in the prenatal period(3/0) were higher than those in control group (0/0)(p<0.05).5The levels of RANTES in eutopic endometrium in mice were higher in the TCDD exposure group (0/3、3/0、3/3、3/10) than those in control group (0/0)(0.2665±0.0104,0.3029±0.0338,0.3286±0.0227,0.4457±0.0463,0.2054±0.0271)(p<0.05); in the group of the mice which received TCDD both in the prenatal and postnatal period, the levels of RANTES were higher in the high-dose exposure group (3/10) than those in the low-dose exposure group (3/3)(p<0.05); the levels of RANTES in the mice which received TCDD only in the prenatal period(3/0) were higher than those in control group (0/0)(p<0.05).6The levels of IL-8in ectopic endometrium in mice were higher in the TCDD exposure group (0/3、3/0、3/3、3/10) than those in control group (0/0)(0.4107±0.0498,0.4360±0.0276,0.5433±0.0389,0.5731±0.0271,0.3125±0.0181)(p<0.05); in the group of the mice which received TCDD both in the prenatal and postnatal period, the levels of IL-8were higher in the high-dose exposure group (3/10) than those in the low-dose exposure group (3/3)(p<0.05); the levels of IL-8in the mice which received TCDD only in the prenatal period(3/0) were higher than those in control group (0/0)(p<0.05).7The levels of RANTES in ectopic endometrium in mice were higher in the TCDD exposure group (0/3、3/0、3/3、3/10) than those in control group (0/0)(0.4183±0.0264,0.4074±0.0351,0.5117±0.0235,0.5404±0.0176,0.3132±0.0176)(p<0.05); in the group of the mice which received TCDD both in the prenatal and postnatal period, the levels of RANTES were higher in the high-dose exposure group (3/10) than those in the low-dose exposure group (3/3)(p<0.05); the levels of RANTES in the mice which received TCDD only in the prenatal period(3/0) were higher than those in control group (0/0)(p<0.05).Conclusions:The levels of IL-8and RANTES in peritoneal fluid, eutopic endometrium and ectopic endometrium were higher in the TCDD exposure group than those in control group. These studies indicate that chemokines involve in the pathogenesis and development of endometriosis which induced by TCDD.