Cytokines SDF-1,RANTES,IL-2 And IL-6 In The Mouse Hippocampus During Pilocarpine-induced Status Epilepticus(PISE)

[Objective]Cytokines SDF-1,RANTES, IL-2 and IL-6 expression in the early epilepsy(SE 30min, SE 1hr,SE 2hr,SE 1day),it used to explore whether the four genes involved in the pathological changes of the early status epilepticus,so that we can better understand the occurrence and development of epilepsy, and better treat it.[Methods]We used 18-20g KM mice which is 4-6 weeks to establish the model of status epilepticus, it was randomly divided into 5 groups, namely(1) control group (injected with saline group),(2) SE 30min group (30min during pilocarpine-induced status epilepticus group),(3) SE 1hr group (lhr during pilocarpine-induced status epilepticus group),(4) SE 2hr group(2hr during pilocarpine-induced status epilepticus groups (5) SE 1day group (24hr after pilocarpine-induced status epilepticus group,its time is longer than 2hr). we made the brain paraffin sections, which is used perfusion to fixed the tissue before doing the brain histology, and used immunohistochemistry to detect cytokines SDF-1,RANTES,IL-2 and IL-6 expression in the hippocampus. We used SPSS 11.5 software package for statistical analysis.[Results] 1. In observation of animal behavior, there were more than gradeâ…£seizures after experimental group mice intraperitoneal injection of pilocarpine; control mice injected with normal saline, no such seizures.2.In the light microscope, SDF-1,RANTES,IL-2,IL-6 immunopositive product was mainly localized in intemeurons of pyramidal cells in CA1-4 areas and granule cells in the dentate gyrus of the hippocampus.3. Immunohistochemical staining showed that,in control mice hippocampus, SDF-1,RANTES,IL-2,IL-6 showed a low levels of expression. 4.Compared with the control group mice, the SDF-1,RANTES,IL-2 and IL-6 which is SE 30min,SE 1hr,SE 2hr and SE lday expression that occurs in mouse hippocampus are increased; its expression which occurs in SE 30mn,SE 1hr to SE 2hr is gradually increase, and it decline in SE 1day, which is lower than SE 2hr, but still higher than the control group,the differences were significant. (P<0.01).[Conclusion] 1.In the light microscope, SDF-1,RANTES,IL-2,IL-6 immunopositive product was mainly localized in interneurons of pyramidal cells in CA1-4 areas and granule cells in the dentate gyrus of the hippocampus. 2. In the control mice hippocampus, SDF-1,RANTES,IL-2 and IL-6 expression of low levels. It showed that they may involved in the normal physiological processes of hippocampus. 3. SDF-1,RANTES,IL-2 and IL-6 expression increased in the early epilepsy, it revealed that four gene involved in the pathological process of initial status epilepticus, and they related to the occurrence and development of epilepsy.