Morphological Changes Of Campylobacter Jejuni Under Different Conditions And Separation Of Campylobacter Jejuni From Faeces

Part1Morphological changes of Campylobacter jejuni under differentculture conditions and biochemical identification by VITEK-2compactObjective：To observe the morphological changes of Campylobacterjejuni under different conditions and the results of the biochemical reactions indifferent turbidity and states.Methods：1Recover Campylobacter jejuni strains stored in the laboratoryand culture them in different culture conditions, then，Gram stain in differenttime to observe the morphological changes;2Recover Campylobacter jejunistrains stored in the laboratory and culture under microaerobic conditions at42℃,5%O2，10%CO2，85%N2.Identfy Campylobacter jejuni strains indifferent turbidity and states by VITEK-2compact and compare to thedifference of biochemical reactions.Results：1Campylobacter jejuni′s shape, when taken from themicroaerophilic environment to the aerobic environment, changes from Gramnegative bacilli to Gram-negative cocci.In a certain period of time,re-passaged back into the micro-aerobic conditions, its shape can be restoredto Gram negative bacilli.But continue to place a longer period of time, itchanges to Gram-negative cocci.2For the same strain of Campylobacter jejuni,other things being equal, the result of identification by VITEK-2compact isdifferent under different turbidity and state.Conclusions：1The morphology of Campylobacter jejuni changes as theculture conditions and nutritional factors;2Campylobacter jejuni′s turbidityand morphology can affect the identification results of the VITEk-2compact. Part2Campylobacter jejuni′s separation from patients′fecesObjective: To explore the separation methed of Campylobacter jejuni infeces from the different groups of people.Methods:1Specimen collection: collect the fresh feces of patients thatnon-Campylobacter jejuni caused disease and of patients with GBS;2Culture:culture the fresh feces with Brucella broth under microaerobic conditions at42℃,5%O2，10%CO2，85%N2. Columbia blood medium culture;3Passaged: for a period of24hours, inoculate to the Colombia blood agar platewith antibiotics, culture, pick suspected single colony passaged;4Identify:use microscopic examination, biochemical test or PCR When needed;5Positive control and quality control: use NCTC11168strain and Lulei strain asthe positive control and quality control strains in the whole process.Results:1In small amount of sample (cotton swab dipped in)group,116cases were collected of from patients that non-Campylobacter jejuni causeddisease, undetached Campylobacter jejuni;collected two cases from patientswith GBS,which is also undetached Campylobacter jejuni;2In largersampling (sampling of about0.5cm3) group,249cases were collected of frompatients that non-Campylobacter jejuni caused disease, undetachedCampylobacter jejuni;collected six cases from patients with GBS, aCampylobacter jejuni strain was isolated, which identified by morphologyand biochemical reactions and PCR.Conclusions:1No Campylobacter jejuni was isolated from patients thatnon-Campylobacter jejuni caused disease. A Campylobacter jejuni strain wasisolated from patients with GBS.2The separation methed of Campylobacterjejuni in feces from the different groups of people was preliminary explored.Myebe Campylobacter jejuni is just as a source of infection instead of thenormal flora of the human intestinal tract; Myebe the methods is not used forthe separation of Campylobacter jejuni in human faeces in this experiment. Part3The preliminary establishment of the database to saveCampylobacter jejuniObjective:1To establish the database to save Campylobacter jejunipreliminary;2To make the database conform to national standards.Methods: Summary of the characteristics of Campylobacter jejuni andbasic research needs projects, and use of computer and Microsoft Excelsoftware,and input the information into form.Results: Campylobacter jejuni information has six parts, Including thebasic information, physiological and biochemical characteristics, drugresistance, genetic information, sharing and other,containing a total of morethan100items.The datebase was divided into six separate form,for eachtable,every Campylobacter jejuni has the same number.Conclusions: The database to save Campylobacter jejuni waspreliminary established.