Morphological Changes Of Campylobacter Jejuni Under Different Culture Conditions And GBS Model Establishment

Part1Morphological changes of campylobacter jejuni underdifferent culture conditions and enterotoxin genesexpression variationObjective: To observe the morphological changes of campylobacterjejuni under different culture conditions and enterotoxin genes expressionvariation.Methods: Culturing the stored campylobacter jejuni strains in differentparterns and conditions. Then, morphological changes of cj was observed byGram stain.Results:1When the condition change from microaerophilic to aerobicenvironment, the shap of campylobacter jejuni began to change from gramnegative bacilli to gram negative cocci at14hours, and totally change to coccishape at30hours. When put campylobacter jejuni into microaerophiliccondition continued, the shape of bacteria began change to cocci at24hoursand totally cocci shaped at48hours.2Culturing these cj in Brucella Broth medium at microaerophiliccondition for84hour, the gram negative bacilli can also be observed.3Put the shape changed gram negative cj which cultured under thecondition of aerobic into microaerophilic condition, the cj shape can return tobacilli shape.Conclusions:1The shap of campylobacter jejuni can change frombacillic to cocci at the condition vary from microaerophilic condition toaerobic condition. And changing time was earlier than culture inmicroaerophilic condition constantly.2Campylobacter jejuni-Lichang can continue to growth at the conditionfrom84hours aerobic culture to microaerophilic condition.3Nutrition condition and state of oxygen are important factors of affect Campylobacter jejuni’s morphology change. Part2Animal model of GBS induced by Campylobacter jejuniObjective: To establish GBS model using Cj infected C57bl/6mouse.Analyze the disease partern of immune-related GBS model. In order to providinformation for further researching of Cj culture and GBS pathogenesis.Methods:1Campylobacter jejuni was isolated from the GBS patients,certified by China CDC. And Campylobacter jejuni serotype was identified toPENO19. Campylobacter jejuni was cultured on Columbia blood agar plateunder mocroaerobic condition at42℃，5%O₂、10%CO₂、85%N₂.2Three immunizing antigen methods were used to model establishing.One is intraperitoneal injection the mixture of MOG35-55and completeFreund’s adjuvant. Two is subcutaneous injection the mixture of MOG35-55,complete Freund’s adjuvant and Campylobacter jejuni. Three is intragastricadministration the mixture of complete Freund’s adjuvant and Campylobacterjejuni. Make the normal mouse as control.3Subcutaneous injection the mixture of complete Freund’s adjuvant anddifferent concentration of campylobacter jejuni.4The clinical signs of mousewere checked daily. That include muscle strength, movement, mental state andso on.5Evaluated the pathology through ＨＥstaining.Results:1Different immune method: At group A,2mouses were foundlimbs paralysis at12-13days after immunity. At group B,2mouses werefound limbs paralysis at14-15days after immunity. At group C,1mouse diedat5days and1mouse totally paralysis. Autopsy showed that blood clot intwo cervical parts and diagnosis as infection.2mouses found bad mental stateand less activity at5-7days after intragastric administration. And thesemouses began paralysis in13-15days. But control group showed good mentalstate.2Different concentration: In high concentration group,6mouses began to occur paralysis signs after11-14days. In moderate concentration group,4mouses began to occur paralysis signs after14-17days. In low concentrationgroup,1mouse found paralysis at16days. There was no mouse paralysis inadjuvant group.3The nerve specimen test showed that axonal degeneration, and nodemyelination.Conclusions: C57BL/6mouse can be made for GBS model andcampylobacter jejuni can induce acute axonal neuropathy.