The Effects Of Probucol Combined With Atorvastatin On The Formation Of Atherosclerotic Plaque And Serum Oxidation Markers

Objective:To observe the effects of Probucol combined with Atorvastatin on the formation of atherosclerotic plaque and serum oxidation markers.Methods:24New Zealand rabbits were divided into Control group (group A, n=6) and High Fat Diet (HFD) group (group X, n=18) randomly. After6weeks, HFD group was divided into3groups by weight randomly.They were High Fat group (group B, n=6), Atorvastatin group (high fat with Atorvastatin5mg/kg.d,qn, group C, n=6) and combined group (high fat with Atorvastatin5mg/kg.d,qn and Probucol0.25g/kg.d.bid, group D, n=6),the drug intervention and high-fat diet were taken simultaneously. The blood fat was examined at the very beginning (0week),6weeks and12weeks. After12weeks, HE and oil red"O" of thoracic aorta, the thickness of endomembrane and tunica media, the area of atheromatous plaque and Immunohistochemical determination of ABCA1and SR-BI levels of blood vessels and liver were examined.158ACS patients (95male patients and63female patients) were divided into the atorvastatin group (n=82) and the combined group (n=76) randomly. At the0week and after12weeks, Ox-LDL and PON1were examined individually. We measured the values before and after the experiment and compared it between the two groups. We also monitored the adverse reaction of drugs during the treatment.Result:1. Animal At baseline(including TC, TG, HDL, LDL), there were no obvious differences (P>0.05) figure1. After6weeks, the blood fat level of group B, C and D was significant higher than that of group A (P<0.05), there was no obvious difference between group B, C and D (P>0.05) figure2. After12weeks, there was difference between group A, B, C and D in TC, TG, HDL and LDL levels. There was significant difference between group C and group D compared with6weeks before in TC, TG, HDL and LDL levels (P<0.05). There was also obvious difference between group C and group D in TC, HDL and LDL levels (P<0.05).2. Compared with group B, there was obvious reduction of thickness of aortic tunica intima and area of atheromatous plaque in group C (P<0.05) and D (P<0.01). There were more expression of ABCA1and SR-BI in group C and D, and the difference between group C and D were obvious (P<0.05). Group D was much more significant.3. ACS patients were divided into the atorvastatin group and the combined group,at baseline of the2groups (including age, gender, BMI, HR, BP, Glu, K+, Cr), there were no obvious differences (P>0.05) After12week’s treatment of ACS patients, the values of Ox-LDL in the combined group were significantly lower than that in the atorvastatin group, and the values of PON1in the combined group were significantly higher than that in the atorvastatin group.4. There was no statistically difference between the two group in safety index (P<0.05).Conclusion:1. There was lower level of TC, LDL and TG in conmbined group. There was effect including reducing area of atheromatous plaque, alleviating inflammatory reaction of plaque and lifting level of ABCA1and SR-BI. It had proved that Probucol combined with Atorvastatin could reinforce fat regulation, strengthen anti-inflammatory action and antioxidation, reinforcing reverse cholesterol transport and benefit reversion of atheromatous plaque.2.In ACS patients, the combined group could reduce the expression of Ox-LDL and rise the expression of PON1more effectively. Probucol can play a more effective role in lipid-lowering, anti-inflammatory and antioxidant, as well as in promoting reverse cholesterol transport.3. The adverse reactions and liver and kidney function damage did not increase in the combined group than that in the atorvastatin group, it proved the safety of the using of the combined medication.