The Effect Of Atorvastatin On The Expression Of (lox-1) Mediated By Oxidized Low Density Lipoprotein(ox-ldl) In Cultured Human Umbilical Vein Endothelial Cells(ecv304)

Background:The statins have been demonstrated to have effective Cholesterol-Reducing Activity and make atherosclerotic plaques regression, thereby, reducing the incidence of clinical coronary heart disease and cardiovascular disease mortality, but the mechanism of its beneficial effects beyound lipid-lowering is yet not clear. Low density lipoprotein(LDL), especially, oxidized low density lipoprotein(ox-LDL) plays a critical role in the process of AS via lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) exerting function.Object:Our present study is aimed to investigate the effect of atorvastatin on the expression of (LOX-1) mediated by oxidized low density lipoprotein(ox-LDL) in cultured human umbilical vein endothelial cells(ECV304).Methods:ECV304 cells were cultured and co-incubated with LDL(25mg/L) and ox-LDL(25,50 and 100mg/L), then the expression of LOX-1 was detected by RT-PCR and Western-blotting, and After treated with atorvastatin(lumol/L and lOumol/L), the mRNA and protein expression of LOX-1 was detected.Results:ox-LDL may mediate the expression of LOX-1 in cultured ECV304 cells and atorvastatin can inhibite the expression induced by ox-LDL.Conclusion:ox-LDL caused endothelial cells damage and atherosclerosis (AS)-like change through induction of LOX-1 expression. The atorvastatin exerted its non-lipid lowering effect in AS treatment via inhibting LOX-1 expression induced by ox-LDL.