Expression And Significance Of Ezrin And Snail In Human Colorectal Carcinoma

Objective:This study aims to test Ezrin and Snail expressions in colorectal carcinoma,normal colorectal tumors and adjacent normal colorectal mucosa throughImmunohistochemistry; to discuss the relationship between expressions of Ezrin andSnail and colorectal cancer clinical and pathological features (such as differentiation,lymph node metastasis, tumor location and type, Dukes stage, blood type, gender andage); and to access the development of colorectal cancer, metastasis and prognosis. Italso provides experimental and theoretical basis to colorectal cancer for diagnosis andprevention.Method:The Immunohistological Envision method was used to detect the expressions ofEzrin and Snail in40cases of human colorectal carcinoma,20cases of normalcolorectal tumors, and20cases adjacent normal colorectal mucosa. The relation-ship of Ezrin and Snail expressions with clinic pathological factors in colorectalcarcinoma was also analyzed.Results:Within the20cases of adjacent normal colorectal mucosa,2cases was foundwith positive expression of Ezrin protein (positive rate10%)；and3cases was foundwith positive expression of Snail protein (positive rate15%). Within the20cases ofnormal colorectal tumors,4cases was found with positive expression of Ezrin protein(positive rate20%)；and5cases was found with positive expression of Snail protein(positive rate25%). With the40cases of human colorectal carcinoma,30cases wasfound with positive expression of Ezrin protein (positive rate75%)； and33caseswas found with positive expression of Snail protein (positive rate82.5%). It showsthe expression of Ezrin and Snail is related to the degree of differentiation ofcolorectal cancer: the rate of positive expression of Ezrin and Snail (88.9%,94.4%) issignificantly higher in the low degree of differentiation group (40%,50%)(p＜0.05);the expression of Ezrin and Snail is related to lymph node metastasis: the rate of positive expression of Ezrin and Snail (86.7%,93.3%) is significantly higher in thegroup with lymph node metastasis (40%,50%)(p＜0.05); the expression of Ezrin andSnail is related to Duke’s stage: the rate of positive expression of Ezrin and Snail(88%,92%)is significantly higher in the group Duke’s C+D than Duck’s A+B (53.3%,66.7%)(p＜0.05).The expression of Ezrin and Snail is unrelated to colorectalcarcinoma gross classification, tumor happening part, patients’ blood group, sex andage(each for P＞0.05). For the group with positive expression of Snail, the ratepositive expression of Ezrin is significantly higher than the negative expression ofSnail group (p＜0.05). There are28cases of patient with both positive expression ofEzrin and Snail, and rate of lymph node metastasis for these patients is70%. Thereare5cases of patient with neither positive expression of Ezrin and Snail, and none ofthem have lymph node metastasis.Conclusion:(1) The higher expression of Ezrin and Snail take in part of generation anddevelopment of colorectal carcinoma.(2) The high rate of expression of Ezrin and Snail in colorectal carcinoma, Therate of expression of Ezrin and Snail in colorectal carcinoma is significantly higherthan that in normal colorectal tumors and adjacent normal colorectal mucos.Inadditional to the relationship between expression of Ezrin and Snail and the degree ofdifferentiation, the lymph node metastasis, and Duck’s stage was closely related tothe degree of malignancy of colorectal carcinoma. It has no relationship withcolorectal carcinoma gross classification, tumor happening part, blood group, sex andage. It might be served as an important factor for evaluating prognosis.(3) The positive correlation was observed between the expression of Ezrin andSnail in human colorectal carcinoma. Combinational examination with both Ezrin andSnail can increase the detection rate of colorectal carcinoma and help to determine thedegree of malignancy of colorectal cancer and in turn to evaluate prognosis.