An Vitro Experimental Study On All Retinoic Acid Enhancing The Bystander Effect Of HSV-TK/GCV Transfected By Ultrasound Microbubbles In SMMC-7721Cell Line | Posted on:2013-04-20 | Degree:Master | Type:Thesis | Country:China | Candidate:W Liao | Full Text:PDF | GTID:2234330374977949 | Subject:Surgery | Abstract/Summary: | PDF Full Text Request | Objective to investigate the bystander effect of HSV-TKtransfected by ultrasound microbubbles in SMMC-7721hepatomacells treated with ATRA.Methods immunohistochemistry was used to survey theexpression of Cx32protein in SMMC-7721cells after treated withATRA;fluorescence microscope and RT-PCR were used to surveythe expression of TK gene transfected by ultrasound waveirradiation in SMMC-7721cells;MTT test was used to survey thechanged bystander effect of SMMC-7721on HSV-TK/GCV aftertreated with ATRA.Results Cx32protein was high expression on cellmembrane but was low expression in cytoplasm, HSV-TK genecan be transfected into SMMC-7721cell by ultrasound waveirraiation and expressed stably;compared with control group,experimental group treated with ATRA enhanced bystandereffect of HSV-TK/GCV on smmc-7721cells(P<0.05).Conclusion ATRA can improve the expression of Cx32proteinand lead Cx32protein to the right location on cellmembrane,which is perhaps correlated with enhancing thebystander effect of HSV-TK/GCV on the SMMC-7721cell line. | Keywords/Search Tags: | Retinoicacid, hepatoma, ultrasoundmicrobubbles, Cx32, HSV-TK | PDF Full Text Request | Related items |
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