Role Of Akt Signal Pathway In Chronically Hypoxic Childern With Cyanotic Congenital Heart Disease

ObjectiveTo explore the effect of Akt signal pathway in chronically hypoxicchildern with cyanotic congenital heart disease.MethodsSamples from the auricle of right atrium were collected from childernwith cyanotic （n=26，SaO₂：60%⁸9%） and acyanotic（n=24,SaO₂>95%）congenital heart disease. The protein expression of Akt and Ser⁴⁷³P-Aktwere examined by western blotting and immunohistochemistry.ResultsThere was no statistical difference between cyanotic and acyanoticgroup in Akt protein expression（p>0.05），however，the protein level ofSer⁴⁷³P-Akt in cyanotic group was significantly higher than that inacyanotic group（p<0.001）and was inversely correlated with preoperativeSaO₂.ConclusionsWe conclude cyanotic hearts adapt to chronic hypoxia through activation of Akt signal pathway. It appears that Akt pathway isresponsible for cardioprotection in the chronically hypoxic children withcyanotic congenital heart disease.