Effects Of MiR-149*on Proliferation,Apoptosis And Invasion Of Bladder Cancer T24Cells In Vitro

Objiec:To obsive the proliferation,invasion and apotosis influence of miR-149*forbladder cancer cells T24occurrence and development of the possible mechanism.Methods:Real-time quantitative RT-PCR,Western Blot T24cells showed lowexpression proteins in microRNA-149*in the T24cells whether differences inexpression.Application chemicalsyn thesis of microRNA-149*mimics,transientransfection of T24cells,respectively by flowcytometry,MTT,transwell experiments;celladhesion expermiments were used to detect microRNA-149*on T24cellapoptosis,proliferation,invasion and cell adhesion capacity.Results:1.sythetic microRNA-149*mimics transfection of T24cells, theconcentration of50Nm miRNA transfected cells when the rate of90%.2.Real-timePCR and Western blot experiments,the results of miR-149*expression in transfectedmiR-149*mimics the group after T24cellsand T24cells,empty vector group andsignificantly themigration of invasion;miR-149*may be increased compared T24cells,indicating that miR-149*T24bladder cancer cells in the differentialexpression.3.flow cytometry,and the results transfected and reduce themigration ofinvasion with miR-149*mimics the T24cells afier their apoptosis and necrosis wassignificantly increased.4.MTT cell count experiments,the results showed thattransfection of miR-149*T24cells fater growth showed,cell proliferation wasinhibited.5.Transwell experiment matrigel line through the cell count showed thattransfection of miR-149*T24cell after in vitro invasion diminished capacity.6.showsthe cell adhesion experiments,transfection of miR-149*T24cells after migration isnot easyto growh,enhanced cell adhesion.Conclusion:1.miR-149*mimics throuth the instantaneous transfection can effectivelyturn to T24cells make miR-149*express rise in the cells2.miR-149*in bladdercancer T24cells showed low expression;vitro transfection of miR-149*after the T24cell proliferation was inhibited to accelerate their apoptosis and reduce the migration of invasion;miR-149*may be involved in human bldder transitional cell cancer anddevelopment.