The Related Research On Apolipoprotein E Genotype In Patients With Cerebrovascular Diseases

Objective1.To investigate the relationship between ApoE genotype in patients withcerebral infarction and intracerebral hemorrhage.2. To explore the relationship between ApoE genotype and neurologic deficit (NIHSSscores).3. To explore the relationship between ApoE genotype and serum hs-CRP level.Methods108patients with cerebral infarction (70cases in acute cerebral infarction,38cases after acute cerebral infarction),78patients with intracerebral hemorrhageand76healthy control group were enrolled. Polymemse chain reaction-restrictionfragment length polymorphism (PCR-RFLP) was used to determine ApoE genotype.The total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol (HDL-C) and hypersensitivityC-reactive prorein(hs-CRP) levels were tested with the automatic biochemicalanalyzer. NIHSS scores in patients with cerebral infarction were scaled withinadmission, one week and two weeks after admission, and within admission in patientswith cerebral hemorrhage admission.Results1The frequency of ApoE ε2and ε4allele account for6.9%and9.7%respectively inpatients with cerebral infarction, while the frequency of ApoEε2and ε4allele accountfor19.1%and3.9%in healthy control. There is a significant difference in thefrequency of ApoE ε2and ε4allele between cerebral infarction group and healthycontrol(P<0.05). The frequency of ApoE ε2and ε4allele account for9.6%and14.8%respectively in patients with intracerebral hemorrhage. There is a significantdifference in the frequency of ApoE ε2and ε4allele between intracerebralhemorrhage group and healthy control(P<0.05).2The TC、TG、LDL-C levels in cerebral infarction group and intracerebralhemorrhage group were significantly higher than that of control group while the HDL-C level was significantly lower than that of control group(P<0.05).3The scores of NIHSS in patients with ε4alleles was significantly increased than thatof patients without ε4in cerebral infarction group(P<0.05). The scores of NIHSS inpatients with ε2alleles was significantly lower than that of patients without ε2inpatients with acute cerebral infarction (P<0.05), while the scores of NIHSS in patientswith ε4alleles was significantly increased than that of patients without ε4in patientswith aute cerebral infarction (P<0.05). The scores of NIHSS in patients with ε4alleleswas significantly increased than that of patients without ε4in intracerebralhemorrhage group(P<0.05).4In patients with acute cerebral infarction, hs-CRP level in patients with ε4alleleswas significantly increased than that of patients without ApoE ε4(P<0.05). In patientswith intracerebral hemorrhage, hs-CRP level in patients with with ε2and ε4alleleswas significantly increased than that of patients without ApoE ε2and ε4(P<0.05)；Conclusion1.ApoE ε4allele might be a susceptible factor for cerebral infarction and intracerebralhemorrhage, and ε2allele be a protective factor for cerebral infarction.2ApoEε4allele might be related to inflammatory disorders duing acute phase incerebral infarction, while ApoEε2and ApoEε4allele might be related to inflammatorydisorders duing acute phase in intracerebral hemorrhage.3. Examination of ApoE genotype, hs-CRP level and NIHSS score may be helpful forprevention of actue cerebral infarction and intracerebral hemorrhage.