The Pharmacological Research On The Active Components Of Fungus Shiraia Bambusicola

Objective:In this work, we tried to separate the active components of Shiraia bambusicola which have the pharmacological effects. Using murine fibroblast L929cell line induced by TNFa as cell model, we preliminarily explored that material bases and molecular mechanisms of the effective components when they regulate cell proliferation bidirectionally. Moreover, the proliferation inhibition of these active components were studied using human gastric carcinoma BGC823as cell model.Methods:(1) The active components of Shiraia bambusicola were separated by Soxhlet extraction and Polyamide column chroma-tography.(2) These components included extA, extB, extC and extD, and the inhibition of the components to the proliferation of L929and BGC823were determined by SRB method;(3) Using Hoechst staining method, we observed the morphological changes of L929and BGC823;(4) Then we detected protein expression level of NF-κB, COX-2, FLIP in L929and NF-κB, FLIP in BGC823by Western Blotting.Results:(1) Combined with the traditional extraction and modern extraction technology, extA, extB, extC and extD were separated from Shiraia bambusicola.(2) When its concentration is between0.03μg/ml-3.75μg/ml, extA effectively antagonized proliferation inhibition of TNFa to L929; while at7.5μg/ml, extA promoted the proliferation inhibition.(3) extA effectively reduced the elevated protein expression level of NF-κB in L929induced by TNFa with timeliness. Besides, extA couldn’t change protein expression level of COX-2, significantly improving the declining expression level of FLIP caused by TNFa, and the trends were positively related to the concentration.(4) extA and extB can inhibit cell proliferation of BGC823effectively, and the trends were positively related to the concentration. Between extA and extB, extB’s ability to inhibit proliferation was stronger.(5) extB can upregulate the protein expression level of NF-κB and FLIP, and the trend was positively related to the concentration.Conclusions:(1) extA and extB of these active components have strong pharmacological effects. Most obviously, extA can bidirectionally regulate proliferation of L929which induced by TNFα. However, extB can significantly inhibit cell proliferation of human cancer cells BGC823.(2) When extA and extB are involved in cell proliferation, some proteins play important roles, such NF-κB, FLIP.(3) extC and extD have no effect on this research.