Expression Of IGF In Hepatocellular Carcinoma Tissue And Paracancerous Tissue And Its Relationship With The Expression Of HTERT

Objective To study the influence of diabetes mellitus on hepatocellular carcinoma through retrospective analysis. To study the expression of IGF-Ⅰ (insulin-like growth factor-1, IGF-Ⅰ), IGF-Ⅱ (insulin-like growth factor-2, IGF-Ⅱ) and telomerase reverse transcriptase hTERT and their relationship in hepatocellular carcinoma and adjacent tissue of patients with hepatocellular carcinoma (hepatocellular carcinoma, HCC). To investigate the role of IGF-Ⅰ and IGF-Ⅱ in the progression of hepatocellular carcinoma.Methods Collect210admission records of liver cancer patients in a hospital and compare the difference of tumour differentiation, neoplasm stage, and alpha-fetoprotein levels between diabetic and non-diabetec group in hepatocellular carcinoma patients. Collect10specimens of hepatocellular carcinomas(HCC) and their surrounding liver tissues from a hospital operation room. Access to all case information of specimens, and make their pathological sections. Detect the expression of IGF-Ⅰ, IGF-Ⅱ and hTERT in hepatocellular carcinomas(HCC) and their surrounding liver tissues of10cases by RT-PCR (Reverse Transcription Polymerase Chain Reaction,), and analyze the correlation between IGF-Ⅰ, IGF-Ⅱ and hTERT. Statistic methods included Student’s Test, Fisher’s exact, χ2exact and Spearman analysis.There is significant difference when P<0.05.Result1.There is significant difference of TNM between diabetic and non-diabetec group in hepatocellular carcinoma patients(P <0.05),but no significant difference of age, sex, differentitation, and alpha-fetoprotein concentrations between them(P>0.05).2. IGF-I mRNA is expressed in both cancer tissue and pericarcinomatous tissue, but there is no significant difference between the two groups(P>0.05). 3. IGF-Ⅰ mRNA is expressed in both cancer tissue and pericarcinomatous tissue, but there is no significant difference between the two groups(P>0.05).4. There is no significant difference of expression of hTERT mRNA between the cancer tissues and pericarcinomatous tissue (P>0.05).5. There is no significant difference in the correlation of the relative expression amount of IGF-Ⅰ mRNA and hTERT mRNA, and no significant difference in the correlation of the relative expression amount of IGF-Ⅱ mRNA and hTERT mRNA in the cancer tissue compared with pericarcinomatous tissue (P>0.05).Conclusion There is no significant difference between the expression of IGF-Ⅰ and IGF-Ⅱ in liver cancer tissue and pericarcinomatous tissue. Also, there is no accompanying relationship between the expression of IGF-Ⅰ, IGF-Ⅱ and hTERT in liver cancer tissue and pericarcinomatous tissue.