| Objective:To evaluate the release of three growth factors, basic fibroblast growth factor(bFGF), transforming growth factor beta1(TGF-β1) and epidermal growth factor(EGF) from platelet-rich fibrin(PRF) clot exudate irradiated with low intensity pulsed ultrasound(LIPUS) to optimize clinical use.Methods:(1)Blood collection was carried out on10healthy volunteers(non-smoker males from22-27years of age).20ml of peripheral blood was collected from each volunteer.Blood samples were taken in10ml glass-coted plastic tubes(without anticoagulant) that were immediately centrifuged at3,000rpm(approximately400g) for10minutes. Leave PRF clots in a sterile metal cup for approximately10minutes to let them slowly release the serum contained therein. LIPUS irradiation was applied to the PRF clot exudate serum.(2)The experiments were subdivided into3groups:PRF irradiate with LIPUS15minutes(L-15min group), PRF irradiate with LIPUS30minutes (L-30min group) and only PRF clot exudate serum as a control group.(3)The concentration of bFGF, TGF-β1and EGF from these samples was quantified by Enzyme-linked immunosorbent assay(ELISA). The results were statistically analyzed with SPSS18.0software.Result:The concentration of the3kinds of growth factors obtained after stimulating with LIPUS were as follows:Statistically significant differences of bFGF between each group, L-15min group(318.60pg/ml) was higher than L-30min group(293.00pg/ml) and Control group(278.20pg/ml)(P<0.05); Statistically significant differences of TGF-β1between each group, L-15min group(7259pg/ml) was higher than L-30min group(6035pg/ml) and Control group(5503pg/ml)(P<0.05);The mean concentration of EGF of Control group, L-15min group and L-30min group were177.87pg/ml,181.80pg/ml and177.91pg/ml, there were no statistical difference between each group (P>0.05).Conclusion:LIPUS may increase concentration of release of growth factors in PRF. PRF combined with LIPUS may widen the clinical application of the PRF in periodontal tissue regeneration. |
PDF Full Text Request