Study On Anti-Obesity Effects Of Shoushentang On The Obese Rats Induced By High Fat Diet

"Shoushentang" is an experiential prescription of Yang Genglu, a practised traditional chinese physician. Its clinical effect on weight loss is significantly, and has no limitation on diet in the application process. Its prospects is attractive. This study further validate the effect of "Shoushentang" on weight loss through animal experiments, observe its toxicity and explore the mechanism. And then provide a basis data for the preparation of the commercialization and further clinical trials.Methods:1. The establishment of nutritional obese model in rats.100female SD rats were used for the study, weighed65～88g. The rats were divided into control group (fed with control diet, n=10) and model group (high-fat diet, n=100). After16weeks,40obese rats were selected for the weight loss experiment.2. Acute toxicity test in mice.4KM mice were used for pre-test,40for the maximum tolerated dose experiment. The mice were orally given dosage of0.8ml/20g body weight of "Shoushentang". The "Shoushentang" concentration is5.33g/ml. Observe7days.3. Effects of "Shoushentang" on the obese SD rats fed with high-energy diet.40obese SD rats were randomly divided into control group, bishengyuan group,"Shoushentang" low-dose group and "Shoushentang" high-dose group. The doses were12.96ml/kg,9.24ml/kg,6.48ml/kg and12.96ml/kg respectively. Daily gavage for4weeks. All rats were maintained on a high-fat diet during the experment. Testing indicators:body weight, food consumption, Lee’s index,9hematological indicators and13blood biochemical indicators.4. Effects of "Shoushentang" on the obese SD rats fed with control diet. All rats aboving were given a control diet for6weeks before the experiment. Then the obese rats were randomly divided into four body weight-matched groups. Grouping, dosage and administration cycle were the same as the aboving3experiments. All rats were offered a control diet during the experiment. Testing indicators:In addition to experimental indicators the same as the aboving3experiments, liver coefficient, fat coefficient, fat cell counting and fat cell area were achievedResults:1. All rats were given a high-fat diet for16weeks. Their body weights showed a tendency to a higher increase. The rats selected gained significantly more body weight than the control group.2, the maximum orally tolerated dose in mice of "Shoushentang" was not less than213g/kg of crude drugs, which was approximately93times higher than the human clinical trial dose(160g/70kg).3."Shoushentang" high-dose promotes weight loss in obese SD rats with a high-energy diet. Serum total cholesterol, LDL and HDL also decreased in the "shoushentang" high-dose group.4. Effects of "Shoushentang" on the body weight in obese SD rats fed with control diet were not obvious. A variety of detection indexes fluctuated within the normal range, the weight loss is not obvious.Results:The results showed that "Shoushentang" promotes weight loss in obese SD rats with high-energy diet (analog of human non-restricted diet in obese patients). Within the dose range used in this experiment, the rats did not show any side effects."Shoushentang" high-dose (12.96ml/kg) could reduce serum cholesterol in high-fat fed rats, this may be one of their weight loss mechanism. The effect "Shoushentang" on weight loss in obese rats was not obvious, and further study was needed to improve effect.