| In our country, cancer is the first cause of death in city residents and the secondcause of death in rural residents. Cancer is the leading cause of death of the mostcommon diseases.Morbidity and mortality are increasing year by year.The tumor isthe body in all kinds of tumorigenic factors under the action of local tissue cells ofcause abnormal growths, by controlling the growth of cells proliferation mechanismand cause disorder disease, its occurrence and development and around the tumorblood vessels are closely related. Vascular endothelial growth factor (VEGF) thatstimulates vasculogenesis and angiogenesis is thought to be an anti-angiogenic targetfor cancer therapy. Many targeting VEGF agents have been developed, but most ofthe current anti-VEGF agents have some side effects such as hypertension，bleeding,gastrointestinal perforation, etc. if they be used chronically.Abundant studies have demonstrated that many flavonoids which exist in plantshave multiple anticancer properties including antiangiogenesis effects. Liquiritigenin(LQ), as a flavanone existed in Radix glycyrrhiza, shows extensive biological activities, such as anti-inflammation and anti-cancer. Our former research indicatesthat LQ can suppress cell proliferation of human hepatocarcinoma (SMMC-7721)cells and human cervical carcinoma (HeLa) cells and induce cells apoptosis via themitochondrial pathway. In addition, LQ can inhibit tumor angiogenesis in humancervical carcinoma (HeLa) cells. In vivo, LQ has a significant inhibitory effect in H22tumor-bearing mice. However, the effects and mechanisms of LQ on antiangiogenesisare not clear in vivo. To discover possible mechanisms of restraining the growth oftumor and its anti-angiogenesis of LQ by way of observing the expression of VEGFand TSP-1on human cervical carcinoma (HeLa) cells xenografts in nude mice. Thisstudy was aimed to elucidate the antitumor and angiogenic effects of LQ on humancervical carcinoma (HeLa) cells xenografts in nude mice. To further clarify themolecular mechanisms of its anti-angiogenesis effects, we detected the angiogenicgrowth factors such as VEGF in anti-angiogenesis to supply evidence for antitumorof LQ. Objective: Establishment of human cervical cancer (HeLa) cells xenografts model innude mice. To study the effects of LQ on inhibition the growth of tumor in humancervical carcinoma (HeLa) cells xenografts in nude mice.Methods：The HeLa cells of logarithm-growth phase, the concentration of cells weresubcutaneous injected into nude mice to establish human cervical cancer (HeLa) cellsxenografts model. Three is32nude mice.Nude mice were divided into4groups,namely, control, three LQ groups with low, middle, high doses (10,20,40mg/kg,respectively). Nude mice were administered intragastrically once per day. Generalreactions were observed every day after drug administration; tumor size and mouseweight were measured by caliper and balance, respectively, every three days until theexperiments were terminated. Mice were euthanized by cervical dislocation at the28th day and Orbital picks, weigh tumor weight, Part of fixed production paraffinsection of formaldehyde, part of liquid nitrogen in preservation.Immunohistochemistry was used to detect PCNA expression on tumors section.Results：Establishment of human cervical cancer (HeLa) cells xenografts model innude mice. Used different doses of LQ（10mg/kg,20mg/kg,40mg/kg）to handle nudemice, the tumor weight compared with the control have reduced and dose dependenteffect. According to nude mice tumor volume rendering of dynamic change thegrowth curve. After all the treatment, the experimental groups compared with thecontrol, tumor growth was muffled, and have dose dependent effect. Human cervicalcancer (HeLa) cells xenografts tumor section showed PCNA expression level byimmunohistochemistry. Statistical analysis showed that, with LQ dose increased, PCNA expression down gradually. During the experiment, the nude mice diet,behavior and mental state have no significant difference. Compared with the control,each group of nude mice has no significant differences on appetite and weight.Conclusion: LQ can inhibit Human cervical cancer (HeLa) cells xenografts tumorgrowth and each group of nude mice has no significant differences on appetite andweight. Objective: To study whether LQ can inhibit the tumor angiogenesis, and to explorethe mechanism of angiogenesis effect of LQ.Methods: Immunohistochemistry was used to detect CD31, SMA, VEGF, TSP-1expression in nude mice human cervical cancer (HeLa) cells xenografts tumorssection; VEGF, TSP-1expression level was measured by ELISA method in nude miceserum; Application of Western blot methods to test the tumor tissue of VEGF, TSP-1the protein expression level in each group of nude mice.Results： Tumor tissue of CD31、SMA protein, VEGF protein expression than that ofthe control group low,but TSP protein expression no change. Statistical analysisshowed that, Compared with the control with significant difference（P<0.01）, and adose dependent on effect. ELISA method detection nude mice serum VEGF/TSP-1the expression level, LQ (10mg/kg,20mg/kg,40mg/kg) group compared with thecontrol VEGF express are down-regulated, Difference was statistically significant (P<0.01). TSP-1express no significant changes show that LQ may through targetsVEGF to suppress the tumor angiogenesis role. Application Western blot methodsdetection tumor tissue of VEGF/TSP-1the protein expression level, and the resultshows that LQ inhibited the tumor tissue angiogenesis in related factors of VEGFexpression, and a certain dose dependent on effects, and the TSP-1to express effect isnot apparent, the results with immunohistochemical and ELISA method areconsistent.Conclusion: LQ can inhibit Human cervical cancer (HeLa) cells xenografts tumorangiogenesis and the molecular mechanism was related to angiogenesis growth factors VEGF. |
PDF Full Text Request