Tumor Necrosis Factor-α And Lymphoma Research

Objective: To investigate the expression of tumor necrosis factor-α (TNF-a)in the lymphoma patient tissues, plasma and the polymorphism of tumournecrosis factor–α (TNF-α) gene atposition-308, which associated with thedevelopment of lymphoma. Methods: collecting97cases of patients oflymphoma in paraffin-embedded tissues and peripheral blood specimens andclinical data. Using Immunohistochemical (SV method) and enzyme-linkedimmunosorbent assay (ELISA) detect lymphoma patients paraffin-embeddedtissues and the level of TNF-a in plasma. Meanwhile using multiple SNaPshotdetect the TNF-a-308sites gene polymorphism of lymphoma patients.Collecting5cases of reactive hyperplasia of lymph node paraffin-embeddedtissues and90cases of healthy control peripheral blood samples as theexperimental control. Compare expression of clinical data, organizations,plasma TNF-a protein, and TNF-a-308genotype and relationship between ofallele frequencies of lymphoma patients. Results:1) The expression indifferent types of lymphoma were difference, While plasma cell neoplasms(PCN) and lymphoblastic lymphoma (LBL), LBL, and peripheral T-celllymphoma (PTL) difference was significant (P<0.05).2) TNF-α levels in theplasma of lymphoma patients were significantly higher than control group[(38.86±26.91) pg/ml vs (33.02±17.22) pg/ml, the difference was statistically(P<0.05);TNF-alpha-308G/G genotype of plasma (38.79±27.81) was lowerthan that G/A genotype (42.44±23.67), the difference was not statistically(P>0.05); plasma level except for PCN and LBL lymphoma patient group werehigher than the control group was significant (P<0.05). Plasma levels ofTNF-a expression in lymphoma patients in male group, Ann Ardor stage IV,high-risk group of the IPI index, symptoms of group B was significantlyhigher than the female group, Ann Ardor stageⅠ~Ⅲ, the IPI index is low risk,group without B symptoms, the difference between the groups was significant (P<0.05). Expression of lymphoma patients in plasma TNF-α and tissues wasnot significantly (P>0.05).3) TNF-a-308G/A locus genotype frequencies inpatients with lymphoma group GA+AA genotype frequency (18.56%) higherthan control group (7.88%), difference was significantly (P<0.05). The Aallele of lymphoma group (9.80%) high than control group (3.94%) differencewas statistically (P<0.05). The gene polymorphism of TNF-alpha-308associated with susctibility to lymphoma, and the individual with AA+AGgenotype, increase the risk of developing NHL, small lymphocytic lymphoma.Conclusion:1) High expression of tumor necrosis factor-a in lymphomatissue and plasma may be associated with the development of lymphoma.2)Detection of TNF-a plasma concentration may more meaningful to evaluateclinical prognosis.Low expression of TNF-a in the LBL and the PCN may berelated to immune function, the detailed mechanism needs further study.3)Locus A allele gene of TNF-α-308may be as a predisposing factor forlymphoma.The gene polymorphisms of TNF-α-308was associated withsusctibility of lymphoma.