Study On Micellar Electrokinetic Chromatography Methods For The Analysis Of Chinese Traditional Medicine

Micellar electrokinetic chromatography (MEKC) is an important separation mode of capillary electrophoresis techniques. It has the advantages of capillary zone electrophoresis (CZE) such as high efficiency separation, rapid separation and analysis, and low consumption of sample. MEKC is suitable for the separation of both neutral and charged compounds. Microemulsion electrokinetic chromatography (MEEKC) is a modification of the MEKC. It has the advantage over MEKC because it is suitable for the analysis of highly hydrophobic solute.In this thesis, the application of MEKC and MEEKC in analysis of Chinese traditional medicine (TCM) was introduced, especially for the simultaneous determination effective components in TCM. The main contents are as follows:1. A MEKC method was established for the simultaneous determination of rutin, danshensu, ginsenoside Rb1, ginsenoside Rg1and notoginsenoside R1in Xiaoshuantongluo Tablet. An uncoated fused silica capillary (75μm×60cm) was used for the separation, and the running buffer was60mmol·L-1SDS-30mmol·L-1Tris-10mmol·L-1H3BO3-15%methanol (pH=8.8). The separation was finished within26min. The detection limits of rutin, danshensu, ginsenoside Rb1, ginsenoside Rg1and notoginsenoside R1were0.30、0.90、3.0、5.0、6.0μg·mL-1, respectively.And the average recoveries of the five components were between93%-108%.2. A MEEKC methods was established for the analysis of paeoniflorin, ferulic acid, salvianolic acid B and tanshinone IIA in Naoxintong Capsule. The compounds was separated on an uncoated fused silica capillary (75μm×60cm), with50mmol·L-1SDS-60mmol·L-1Tris-8.3%n-butanol-1.3%hexane-15%acetonitrile (pH=8.8) as the running buffer. The detection limits for these four compounds were1.5,0.50,2.0,1.0μg·mL-1., respectively. The average recoveries of the four components were between92%-104%.3. A MEEKC method was established for the separation of prim-O-glucosylcimifugin,4’-O-glucosyl-5-O-methylvisamminol, emodin and rhein in Toutongning Capsule. The best separation was obtained with40mmol·L-1SDS-10mmol·L-1Na2B4O7-1.3%chloroform-10%methanol (pH=9.2) as a running buffer. The influence of different alcohol as cosurfactant on the separation of compounds was investigated. And the detection limits of four compounds were0.30,0.10,0.30,0.20μg·mL-1, respectively. The average recoveries of the four components were between92%-102%.4. Simultaneous determination of Puerarin, ferulic acid, scutellarin, danshensu, tanshinone IIA in Dandengtongnao Capsule method was established by MEEKC with SDS and lauric acid as a mixed micelles. The running buffer was40mmol·L-1SDS-15mmol·L-1lauric acid-10mmol·L-1Na2B4O7-1.3%hexane-15%methanol (pH=9.2). The detection limits of these five compounds were0.20,0.50,0.50,0.60,1.0μg·mL-1, respectively. The average recoveries of the five components were between90%-104%.