Study On Preparation Of Camellia Chrysantha Polyphenol Hpmcp Microcapsule And Its Antioxidant Capacity

In this paper, TPs-HPMCP microcapsule were desired to fully utilize the rare resource of Camellia chrysantha(Hu) tuyama and avoid the damage by the gastric fluid. The coarse polyphenol extractions of Camellia chrysantha were purified with macroporous resin,and HPMCP was used as wall material, TPs-HPMCP microcapsule were prepared by spray-drying method.The research focused on several aspects as follows:1. Study on purification of coarse polyphenol extractions from Camellia Chrysantha with macroporous resinThe coarse polyphenol extractions of Camellia chrysantha were purified with XAD-16macroporous resin and eluted by ethanol in different concentrations and gradients.The results show:when the velocity of elution is4BV/h,the highest purification of Camellia chrysantha which was eluted by60%ethanol is80.19%. We can separate and purify the polyphenol of Camellia chrysantha by XAD-16macroporous resin as immobile phase and eluting with ethanol in different concentrations and gradients as eluent.2. Preparation of TPs-HPMCP microcapsuleThe microencapsulation of TPs was prepared through spray drying technology with HPMCP as wall stuff and optimized by orthogonal design L9(34) with the encapsulation efficency of microcapsule as the index. Addition of emulsifier (SDS) corrupted4%gross weight of core and wall materials. The optimized processing parameters of TPs-HPMCP microcapsule were that entrance temperature was80℃,entrance speed was6.2ml/min, ratio of TPs to wall material was2:1, total solid concentration was2%.3. Study on antioxidation of TPs-HPMCP microcapsule and its in-vitro releaseThe study indicated that HPMCP could protect the TPs in the gastric fluid, and TP-HPMCP microcapsule released perfectly in the intestinal fluid.The antioxidation of TPs microcapsule (specimen A) and TPs-HPMCP microcapsule (specimen B) were studied by four different systems(·OH、 DPPH·、O2-、NO2-),using green tea PolyPhenol as positive controls. The results showed that TPs-HPMCP microcapsule had obviously scavenging activity. The antioxidation ability of sample A and sample B was similar to green tea PolyPhenol4. The study of microcapsule storage stabilityThe storage stability study on Camellia chrysantha TPs-HPMCP microcapsule and original Camellia chrysantha microcapsules showed that the retention rate of tea polyphenol in Camellia chrysantha TPs-HPMCP Microcapsules is81.23%while the other is only45.56%after20days, which declined that HPMCP microcapsules protected the internal core material more effective and had good stability.