Effects Of Niflumic Acid On The Expression Of C-fos In The Proliferative Rat Airway Smooth Muscle Cells In Vitro

Objective: To investigate the effects of NFA on the expression of c-fos inproliferative rat airway smooth muscle cells.Methods: Rat airway smooth muscle cells(ASMCs) were isolated andcultured in DMEM containing15%fetal bovine serum, and passage3～5cellswhich grew well were used in the experiment. Next the cells were cultured inDMEM medium containing different concentrations of NFA(0、10、50、100umol/L) for1、2、3hours. Then total RNA of ASMCs was isolated, and thec-fos gene expression levels were detected by reverse transcription polymerasechain reaction (RT-PCR).Results: The c-fos gene expression levels of ASMCs in high, middle andlow concentration of NFA groups were significantly lower than those incontrol group. With the increase of NFA concentration, the expression of c-fosdecreased. Conclusions: Niflumic acid could significantly inhibit the geneexpression of c-fos in proliferative ASMCs.