The Correlate Research Of Low Concentration Metformin Act On Pancreas Tumor Cells

Objective:To observe the function which metformin with different concentrationplayed on pancreatic tumor cell lines（ASPC1,BxPc3）and coordinate functionbetween gemcitabine, and trying to illuminate the cytocide effect of low concentratemetformin （≤0.1mM）act on CD133+subgroup cells.Methods:We use lower concentrate metformin （≤0.1mM）to treat pancreatic tumorcell lines（ASPC1,BxPc3）and testify the function on cell growth by CCK8kit andcell count respectively at12,24,36,48,60,72hour, then according the resultsdrawing cell growth curve. Identify CD133+subgroup by cytoflowmetry to measurethe effect of lower concentrate metformin（≤0.1mM）act on tumor stem cells. AddingAMPK cell signal inhibitor(Compound C) to explore molecule mechanism.Results:Low concentration metformin （≤0.01mM） have not any statisticdifferences(P＞0.05)on pancreatic tumor cell growth, by oppose, high concentrationmetformin （≥1mM） own obvious cytocide effect(P＜0.01). Effect of lowconcentration metformin（≤0.01mM）on CD133+group cell got statistic differences(P＜0.05). Beside, low concentration metformin can increase the sensitivity ofASPC1,BxPc3to therapy dosage gecmitabine. Adding Compound C did not disturbthe effect of low concentrate metformin much（P＞0.05）.Conclusion:Effect of metformin act on pancreas tumor cell linear(ASPC1,BxPc3)correlated with its concentration. However, low concentration metformin can increasethe sensitivity of ASPC1,BxPc3to therapy dosage gecmitabine and selectively killCD133+subgroup cells which do not much mechanical dependability withAMPK/mTOR.