Relationship Between Gene Polymorphisms Of Vascular Endothelial Growth Factors+405G>C And-2578C>A And Bronchopulmonary Dysplasia

Objective To investigate the association between single nucleotide polymorphismof vascular endothelial growth factor (VEGF) and bronchopulmonary dysplasia (BPD).Methods Genotype frequency and gene frequency of VEGF polymorphisms were performedby polymerase chain reaction and restriction fragment length polymorphism analysis(PCR-RFLP). Enzyme-linked immune-sorbent assay (ELISA) was used to detect theexpression level of VEGF in plasma in BPD group. A total of57BPD patients in BPD group and126patients without lung disease in control group from july1,2009to february1,2012were included in TongJi Hospital in the study. Results①VEGF+405G>C: Compared withcontrol group, the genotype and allele frequencies of G was higher in BPD group withstatistical difference (p<0.05). VEGF-2578C>A: Compared with control group, the genotypeand allele frequencies of A was higher in BPD group with statistical difference (p<0.05).②In BPD group the level of VEGF+405was lower in the patients with genotype GG(p＜0.05)compared with CC while there was no difference between VEGF-2578with genotype CCand GG (p>0.05). Conclusions Single nucleotide polymorphisms of VEGF+405andVEGF-2578could be associated with BPD and VEGF+405influences its proteinexpression, and the lower VEGF level in plasma could be one of the factors for BPD genesusceptibility pathogenesis.