The Level And Significance Of MIF And PLTP Expression In Bronchopulmonary Dysplasia Mouse Serum

ObjectiveExposure to oxygen (FiO2:65%) to establish the mouse model of bronchopulmonarydysplasia.Through the establishment of mouse models of BPD,to study the level andsignificance of MIF and PLTP expression in BPD mouse serum,to seek the Pathogenesis ofBPD,Provide a new laboratory foundation to its diagnosis and treatment.Methods120Kunming4-day-old clean-type newborn mice were randomly divided into twogroups(oxygen group and air group,=60),either male or female.Oxygen group exposed inthe oxygen tank(FiO2:65%),the air group was exposed to air (under the standardatmospheric pressure).In the first seven days,14days,21days,28days of the experimentobserve the general condition of the animals and weight.Blood samples were drawn byeyeball method,using the method of enzyme linked immunosorbent assay(ELISA) to detectthe content of MIF and PLTP.Separation of lung tissue with HE staining,observing themorphological changes of the lung tissue, measuring the thickness of the respiratorymembrane and calculatiing the radial alveolar count.Using SPSS13.0for statistical analysis.Results1.The general condition and weight of the animal:With the continuous extension of theoxygen time,the mental of the rates gradually deteriorated,unresponsive to stimuli andreduced activity,and gradual emergence of dyspnea and cyanosis.The air group eating well,ponderal weight stable,having no difficulty in breathing and cyanosis.2.Stained by HE,the lung Parenchyma show markedly thickened alveolar septa withmajorseptal fibrosis and some muscle fibres associated with vascular changes(intimalproliferation,duplication of the elastic laminate,medial hyperplasia,and arterialisation of theveins)on the21days after experimen(P＜0.01).Lung tissue can be seen small pieces of hyperinflation,fibrosis,emphysema of the region.Focal squamous metaplasia of the airwayepithelial cells,and bronchial mucous glands dry up.3.ELISA resultsPLTP level were higher than the control group in the first and second week after birth,and then decreased significantly. PLTP level gradually increased to the highest level afterbirth,reached the highest level at the third week and beganed to decrease at the fourth week.But higher than the level of the third week of the oxygen group after birth.The content of MIF were significantly higher from the beginning of the expe-riment,with the extension of the oxygen time,the level was significantly higher than thePeers air mice.At the time of thied week the mice have severe oxygen dependent,underdetachment oxygen status mice can be severe difficulty in breathing,histological andmorphological alveolitis and fibrosis reached the highest value,at this time MIF levelreached a peak in the experimental group.This shows that lung injury has been increasingwith the extension of the oxygen time,in other words,MIF play an important role in theformation of hyperoxia-induced lung injury and bronchopulmonary dysplasia.Conclusions1.Continuous65%oxygen exposure can lead to the formation of newborn mice BPDmodel,the process is very similar to human preterm children of BPD development.2.The level of PLTP incresed in acute lung injury period can be used as a sensitiveindicator and early change of acute lung epithelial cell damage and pulmonary capillaryinjury.Later in experiment the PLTP level reduced,it can used as a indicator of the finaldegree of lung injury and the long-term prognosis of lung injury.MIF level was significantly higher in the oxygen group,indicating that its levels may beassociated with the occurrence of BPD was positively correlated,that its levels maybePositively correlate with BPD occurred.3.The expression level of MIF and PLTP may provide better ways for the earlydiagnosis of BPD,open up new ideas for the prognosis and prevention of BPD.