Expression Level And Significance Of Serum Clara Cell Secretory Protein In Bronchopulmonary Dysplasia Mice

ObjectivesTo investigate the influence of newborn mice lungs develop in the concentration ofoxygen through the mice bronchial lung dysplasia (Bronchopulmonary dysplasia, BPD)model which had exposed to the concentration of oxygen (60%), and test the serum levelexpress CCSP of these BPD mice, in order to provide a new path for earlier diagnosis,treatment and forecast for BPD.Methods120clean freshmen kunming mice which were4days old, were randomly dividedinto oxygen group and air group with60mice respectively. The oxygen group were lied inthe box with oxygen concentration of60%to make BPD model, and the air group werelied in the air box.12newborn mice were selected randomly from both oxygen group andair group at the7th day,14th day,21st day and28th day. All mice were weighted, and theneyes were picked up to gain blood specimens. We detected the level of serum CCSPexpression through application elisa method, and separated lung tissue to observe lungtissue morphological changes after HE dyeing. And we measured the thickness of the filmbreathing through the calculation of radial alveolar count (RAC) that can show the numberof alveolar, and through the lung tissue fibrosis scores double-blindly.Results1. General condition: mice in the air group with thicker subcutaneous fat,were activeand well developed; but mice in the oxygen group with dry skin, were angular, depressed,lazy, with stunted growth, cyanosis and shortness of breath.2. Morphological changes of lung exposed to the concentration of oxygen at the21stday: the alveolar space of mice increased significantly with alveolar fusion, the normalalveolar structure disappeared,the radial alveolar count reduced (P <0.01), and lungmesenchymal cells and collagen increased significantly (P <0.01), and the breathing membrane was thicker (P <0.01).3. The level of serum CCSP in different time points has no significant change in airgroup,which fluctuated between14.97and17.54(P>0.05). The expression level of serumCCSP of oxygen mice at14th day is significantly higher than those in air group (P <0.01).With the extension of time, the oxygen at21st day and at28th day in the expression levelof serum CCSP significantly lower than those in the air group (P <0.01), and significantlybelow the level of the same group at the14th day (P <0.01).Conclusions1. Long-term inspiratory of60%concentration oxygen can cause lung tissue damageand lung development block of newborn mice, lead to alveolar developmental disordersand lung tissue fiber hyperplasia, which has the same characteristics of human BPDpathology.2. The dynamic CCSP changes can reflect the lung inflammation which can serve asone of the important indexes for early diagnosis of BPD.