Pannxin-1Protein Expression And Significance Of Refractory Epilepsy In Rat Hippocampus

Epilepsy is a common and frequently occurring diseases of the nervous system, it isfrom brain neurons to unexpected short-term highly synchronized abnormal discharge,Lead to short-term brain dysfunction, a chronic neurological diseases, and it’scharacterized by recurrent, transient, repetitive, stereotyped. Majority of patients withepilepsy taking antiepileptic drugs to control seizures, but there are still some patientstreatment is not ideal, is called medically intractable epilepsy. Pathogenesis of epilepsy isstill not very clear, has been a hot topic of epilepsy research in the field. studies suggestthat gap junction channels may play an important role during the development of epilepsy,Panx1is half channel protein gap junction, The main function may be abnormalsynchronous firing mediated between neurons and ATP and Ca²⁺wave passed involved inapoptosis of neuronal cells. In this experiment we will observe the change and variationPanx-1protein expression in rat brain tissue in refractory epilepsy, and to Explore theeffect and mechanism of Panx-1protein in the development of epilepsy, Looking forpossible avenues for the treatment of epilepsy and control.Method:1. The establishment of animal models：Selection of the normal adult male Wistar ratswere randomly divided into normal control group （n=6） with the model group （n=32）,The successful firing of the model group was divided into three subgroups,24h,10d,21dfor the study time points, The model rats by intraperitoneal injection of Lithium-Pilocarpine, Control group by intraperitoneal injection of saline, according to the Racineepilepsy classification standards, up to the IV or more included in the experiment.2. Observation of the rat’s behavior changes daily. Normal control group, and seizuresafter24h,10d,21d rat ambulatory EEG detected respectively.3. Each rat brains were removed according to the time point, using of PCR, Westernblot, immunohistochemistry and other methods to observe the law of the Panx-1protein expression changes in rat model of epilepsy, and further explore Panx-1’s role in thepathogenesis of refractory epilepsy.Result:1. Lithium–Pilocarpine induced rat model of epilepsy, success rate was84%and themortality rate was11%, After12.5±3.5d, can be observed in rat spontaneous seizures,EEG in the acute phase of performance for the sharp spikes of high frequency ratevolatility, stationary, not found in the epileptic, only the performance of the slowing downof the background wave volatility increased; Chronic （21days）, EEG monitoring foundthat periodic epileptiform discharges.2. PCR、Western blot and immunohistochemistry showed that the hippocampusPanx-1protein at each time point of expression in the model rats were higher than normalcontrol group （p <0.05）, SE24h increased most significantly （p <0.01）.Conclusion:1. LICL-PILO epilepsy rat model is the most effective model of refractory epilepsy.2. Panx-1protein in the hippocampus in the rat model of refractory epilepsy,increased expression of acute phase, was gradually decreased over time.3. Panx-1may play an important role in the occurrence and development of refractoryepilepsy.