Initial Involvement Of Curcumol In Regulation Of Apoptosis And Proliferation In Human Gastric Cancer And Implicated Mechanisms

Objectives:To initially investigate the mechanisms of the effects of curcumol on the proliferation and apoptosis of human gastric cancer SGC7901cells.Subject:Human gastric cancer SGC7901cellsMethods:Curcumol was dissolved in dehydrate alcohol and initial concentration of curcumol was5mg/mL. Group1、2、3、4and5as experimental groups were set up. The curcumol concentrations were set as follows:5μg/mL、10μg/mL、20μg/mL、40μg/mL、80μg/mL (the experimental groups contained1%dehydrate alcohol) Control group contained1%dehydrate alcohol. After cultured for48h, the Trypan blue method was used to analyze the cytoactive of SGC7901cells. MTT assay was performed to study the inhibitory rate of SGC7901cells proliferation. The effect of curcumol on SGC7901cells apoptosis rate were detected by FCM. The expression of Bax、Bcl-2、 EKR、 AKT、 p-ERK、 p-AKT were detected by Western blot.Results:1. The Trypan blue dyeing showed that the viable rate of SGC7901were more than90%, and fit the requirement of experiment. The inhibitory effect of curcumol on proliferation in SGC7901cells treated by5μg/mL for48h is not significant.Comparing to control group,there was not significant (P>0.05). While there was significant (P<0.05) in SGC7901cells treated by10μg/mL、20μg/mL、40μ g/mL、80μg/mL for48h, with the increase of curcumol concentration, the inhibition was reinforced.2.20μg/mL、40μg/mL、80μg/mL curcumol could induce apoptiosis of SGC7901cells after treated for48h detected by FCM assay.With the increase of curcumol concentration,the apoptosis rate was heightened.Comparing to control group, there was significant(P<0.05).While there was not significant (P>0.05)in SGC7901cells treated by5μg/mL、10μg/mL for48h.3.20μg/mL、40μg/mL、80μg/mL curcumol could decrease the expression of Bcl-2and increase the expression of Bax in a dose dependent manner,comparing to control group, there was significant (P<0.05), but there was no significant (P>0.05) in SGC7901cells treated by5μg/mL.10μg/mL for48h.4. Comparing to control group,there was not significant (P>0.05) in SGC7901cells treated by different concentration of curcumol for48h on the expression of ERK and AKT. While curcumol concentration could reach20μg/mL, comparing to control group, there was significant decrease (P<0.05) in SGC7901cells on the expression of p-ERK and p-AKT. Curcumol could decrease the expression of p-ERK and p-AKT, with the increase of curcumol concentration, the inhibition was reinforced.Conclusion:1.20μg/mL-80μg/mL curcumol could inhibit proliferation and induce apoptosis of SGC7901cells through regulating the expression of apoptosis protein in a dose dependent manner. The effect of this study show that curcumol resisted cancer probably through regulating the balance of proliferation and apoptosis in SGC7901cells.2.20μg/mL-80μg/mL curcumol inhibited the activation of MAPK/ERK and PI3K/AKT signal pathway in SGC7901cells.The result of this study show that curcumol probably regulate the balance of proliferation and apoptosis in SGC7901cells,through regulating signal transduction pathway.