Study Of The Relationship Between SFRP2Gene Promoter Hypermethylation Status And Invasion And Metastasis Of Colorectal Carcinoma

Purpose To analyze the correlation of secreted frizzled-related protein2gene hypermethylation of colorectal cancer patient with UICC stages and histological types, and to discuss the potential of secreted frizzled-related protein2gene hypermethylation as a reliable biomarker in invasion and metastasis of colorectal cancer.Subjects SFRP2gene hypermethylation of colorectal cancer tissue and matched tumor-adjacent tissue.Materials and methods1) collection of colorectal cancer tissue samples matched tumor-adjacent tissue samples respectively, then placed them in-80℃low temperature refrigerator.2) Methylation-specific PCR and real-time PCR were used to detect DNA methylation of the SFRP2gene in colorectal carcinoma samples and matched tumor-adjacent tissue samples respectively.3) Statistical analysis:the use of SPSS17.0software for statistical analysis.Results SFRP2gene promoter hypermethylation was occur in both the tumor tissues and the adjacent tissues. The level of SFRP2gene promoter hypermethylation was significantly higher in the tumor tissues than in the adjacent tissues(P<0.05). The level of SFRP2gene promoter hypermethylation was associated with TNM stage(P<0.05), the cell differentiation(P<0.05), depth of tumor invasion(P<0.05), lymph node metastasis (P<0.05)and tumor metastasis(P<0.05). No significant association was found between the level of SFRP2gene promoter hypermethylation and the status of gender, age, tumor location and tumor size.Conclusion SFRP2gene hypermethylation in tissues may be a useful objective parameter for the malignant level, invasion, metastasis, recurrence and prognosis of the colorectal cancer.