Correlative Study Of Telomerase Amplification And Expression With HPV Infection Between Minority And Han Women With Cervical Cancer From Two Regions Of Chinese Guizhou Province

Objective:This study was aimed to investigate the occurent discrepancy between minority (including Miao, Dong, and Tujia) and Han women with cervical cancer from Tongren and Southeast Qian regions in Guizhou, China, and to explore the possibility whether the combined detection of hTERC copy number and hTERT expression could be used for early diagnosis of cervical cancer, which will provide theoretical evidences for more effective screening of early cervical cancerous diseases in these regions.Methods:Tissue microarray was constructed from338paraffin-embeded cervical tissues of patients diagnosed with squamous cell carcinoma. The amplification of hTERC and the expression of human telomerase reverse transcriptase (hTERT), HPV16/18E6, and HPV16E7were examined by FISH or immunohistochemistry. The differences between the results from minority and Han women were computed and analyzed for statistical significance.Results:The results showed that no statistical significance was reached between minority and Han women in terms of the amplification of hTERC and the expression of hTERT, E6or E7(P>0.05). In both minority and Han women, there was no significant difference between the amplification of hTERC and the expression of hTERT (P>0.05), but a high statistical significance was reached between the expression of HPV16/18E6and the amplification of hTERC or the expression of hTERT (P<0.01).Conclusion:With respect to the amplification of hTERC and the expression of hTERT and HPV16/18E6or E7proteins, no significant difference was found between minority and Han women with cervical cancer. However, a significant correlation was found between HPV16/18infection and hTERC amplification or hTERT expression. Thus, the detection of HPV16/18E6and hTERT combined with the examination of hTERC copy number in cervical tissue may increase the screening accuracy of early cervical cancers in both minority and Han women in Guizhou Province, which may be used for early screening of cervical cancer patients.