Genomic Amplification Of Human Telomerase Gene (hTERC) In Cytologic Specimens Of Cervix By FISH

Objectives:1 To evaluate the optimal sampling and processing procedures for dual-color interphase fluorescence in situ hybridization (FISH) studies of the human telomerase gene(hTERC) on the cytology samples of cervix.2 To investigate the expression of the human telomerase gene in the cervical intraepithelial neoplasia,cervical carcinomas and normal,and to evaluate the clinical implications of the human telomerase gene.3 To discuss genomic amplification of the human telomerase gene(hTERC) in cervical intraepithelial neoplasia(CIN) with high-risk human papillomavirus(HR-HPV) infection.Methods:1 The cervical specimens from 282 outpatients,Three groups were divided based on the different sampling and processing procedures,including cervical Pap smears(15 cases),TCT sliders(205 cases),hypotonic sliders(62 cases).The fluorescence signals in cytololgical samples of the cervix which detected by using interphase fluorescence in situ hybridization with the hTERC probe were compared among the groups to determine the optimal procedure. 2 305 cervical samples were divided five groups,including normal,ASCUS,ASC-H,LSIL,HSIL,115cases of Histology biopsy results are CINⅠ,CINⅡ,CINⅢ/SCC,all of these samples were detected the expression of hTERC gene.At some time,the results were compared with 20 samples of normal.3 The 115 samples were detected the high risk HPV-DNA with HC2.analysis the relationship between HR-HPV infection and expression of hTERC gene in different cervical intraepithelial neoplasia lesions.Results:1 The successful rates on visualization of chromosome 3q copy number are different among the three methods.The successful rates is 74.4%in 282 cases,cervical Pap smears,TCT sliders, hypotonic sliders were 53.3%,81.9%,51.6%respectively,The hybridization successful rates in the TCT sliders was significantly higher than others(p＜0.05).2 The expression almost of 2 sign in the normal squamous cervical cells,while there was presented a gain of hTERC gene in the CIN.The percentage of amplification hTERC gene in 305 cases cervical samples of normal,ASCUS,ASC-H,LSIL,HSIL were 8.5%,11.3%,25.8%,41.0%,77.4%respectively,the ASC-H,LSIL,HSIL of cervical cell show extra copies of hTERC when competed with normal,the result show that HSIL lesions could be distinguished from normal samples,the 115cases of CINⅠ,CINⅡ,CINⅢ/SCC were 7.3%,38.5%,63.2%,78.6%.the result show that CINⅢ/SCC lesions could be distinguished from normal samples.3 The sensitivity and specificity of hTERC amplification in CIN were 67.56%,92.68%,wheras,the sensitivity of our test for predicting progrrssion HSIL/SCC was 78.57%and the specificity was 92.68%.4 there was presented a gain of hTERC gene in the CIN and the samples of HR-HPV infected,the percentage of cases with infection of HPV in CINⅠ-Ⅲwas 80.0%,64.7%,80.0%) respectively;the positive rate of cases with amplification of hTERC gene in 62 cases with HPV infected was 75.8%,whereas 11cases without HPV infected was 25.0%,there was statistical significance(P＜0.05).Conclusion:1 When detected the hTERC gene by dual-color fluorescence in situ hybridization technique,the hypotonic sliders is a quick, effective and economical method,and also generate the successful rates,it is suitable for cytological analysis of the cervical cells.2 The expression increase of hTERC in the squamous cervical carcinoma and cervical intraepithelial neoplasia.The 3q copy numbers are associated with the severity of cytologic and histological finding,the hTERC may help to determine the progressive potential of individual lesions.3 Lesions of CIN and HPV infection may be associated with amplification of hTERC gene of chromosome which detected by fluorescence in situ hybridization.