Investigation For DC-CIK Cells Reversing K562/ADM Multidrug Resistance

ObjectiveThis research aims to investigate the effects and mechanism of DC-CIKcells reversing multidrug resistance of K562/ADM cells in vitro,and itscytotoxicity against K562/ADM cells.Methods1. peripheral blood mononuclear cells (PBMNCs) were isolated from healthyhuman Peripheral blood and induced into CIK,DC and DC-CIK cells bykinds of cytokines.The cell morphology was observed undermicroscope;and immunophenotype was detected by flow cytometry.2. The multi-drug resistance gene expressions on the K562cells,K562/ADMcells, K562/ADM+CIK cells and K562/ADM+DC-CIK cells were detected byRT-PCR.3. The sensitivity to ADM in the groups was detected by MTT method.The ODvalue for K562cells, K562/ADM cells, K562/ADM+CIK cells and K562/ADM+DC-CIK cells were measured by microplate reader.Results1. DC and CIK cells were induced purified highly by PBMNC. Theimmunophenotype of cells were showed higher rate by flow cytometry.2. Both MDR1of K562/ADM+CIK cells and K562/ADM+DC-CIK cellsexpressed lower than K562/ADM cells obviously（P﹤0.05）,but the MDR1of K562/ADM+DC-CIK cells express the lowest.3. The sensitivity to ADM for K562/ADM+DC-CIK cells was higher thanK562/ADM+CIK cells greatly（P﹤0.05）,but both of them were higher thanK562/ADM cells（P﹤0.05）.Conclusions1. The highly purified,well vitality DC and CIK cells were induced by PBMNCconveniently.The immunophenptypes of DC and CIK detected by flowcytometry were reliable.2. CIK cells and DC-CIK cells all can reverse MDR of K562/ADM cells,and theeffects of DC-CIK cells were stronger, its mechanism may be relevant tothe decreasing of the mdr1mRNA expression.3. Both DC-CIK cells and CIK cells can increase the MDR cells sensitivity ofADM,and the former is higher obviously.